糖尿病小鼠胰岛移植部位的实验研究

Experimental study on transplantation of mice islets to different sites of diabetic mice

目的探讨小鼠肝内、肾包膜下等不同部位胰岛移植物生存时间及免疫学功能的差异。方法采用滤网法分离纯化胰岛,供体胰岛植入受体肝内及肾包膜下。用酶联免疫吸附试验 (ELISA)测定INF-γ的含量。3H-胸腺嘧啶脱氧核苷(3H-TdR)掺入法测定单向混合淋巴细胞反应。结果肾包膜下移植组胰岛生存时间(12．18±1．25)d明显长于肝内移植组[(7．09±0．70)d,P< 0．01]。单项混合淋巴细胞反应显示,肝内移植组淋巴细胞较肾包膜下移植组增殖明显(P<0．01)。上清液测IFN-γ含量,肝内移植组明显升高,差别有统计学意义(P<0．05)。结论不同移植部位, 胰岛的生存时间及免疫功能差异存在统计学意义,肾包膜下是胰岛移植的理想部位。

Objective To evaluate the ideal site of islets transplantation, both the survival time and immunological function of islets transplanted into the liver and the kidney subeapsule were compared. Method cell strainer to purify islets were purified by using cell strainer and transplanted into kidney subcapsule or liver. IFN-γ was measured by enzyme-linked immunosorbent assay （ELISA）. Tritiated thymidine （^3H-TdR） was used to measure mixed lymphocyte response. Results BALB/C islet allografts transplanted into the kidney subcapsule of C57BL/6 recipients survived significantly longer [ mean survival time, （12.18±1.25） days] than those transplanted into the liver of C57BL/6 [（7.09±0.70） days, P 〈 0.01 ]. Mixed lymphocyte response assay revealed that lymphocytes from liver transplanted recipients exhibited high degree of proliferation comparable to that of kidney subeapsule transplanted recipients （P 〈 0.01 ）. IFN-γ was significantly higher in liver transplanted recipients than kidney subeapsule transplanted recipients （P 〈 0.05）. Conclusion There are marked differences with regard to the survival time and immunological function of islets transplanted to different implantation sites. It was conclude that the renal subeapsular space is an excellent islet transplantation site.