养血清脑颗粒对自发性高血压大鼠(SHR)肾素-血管紧张素-醛固酮的影响

Effect of Yangxue Qingnao Grain on Renin-angiotensin System of Spontaneously Hypertensive Rats(SHR)

目的:通过研究养血清脑颗粒对自发性高血压大鼠(SHR)循环和肾组织局部RAS活性的影响,探讨其作用机制和靶点。方法:60只SHR大鼠随机分为模型组,卡托普利组,牛黄降压丸组,养血清脑颗粒高、中、低剂量组,共6组,同时选取10只WHY大鼠作为对照组。给药8w后,用仪器SN-695B型放免γ测量仪以放免的方法测定血浆和肾脏组织内血管紧张素Ⅱ(AngⅡ)、醛固酮(Ald)。结果:使用各组药物后,肾组织血管紧张素-醛固酮系统变化显著,养血清脑颗粒中剂量组AngⅡ含量和模型组相比有显著差异,P<0.001;高剂量组、低剂量组和模型组相比,也有显著性差异P<0.05。养血清脑颗粒高、中剂量组醛固酮含量比模型组明显降低。结论:养血清脑颗粒很可能是通过在肾组织内血管紧张素-醛固酮系统发挥其降压作用和肾脏保护作用的。

Objective：To Observe the effect of Yangxue Qinnao grain on plasma and kidney tissue renin-angiotensin system （ RAS ） of spontaneously hypertensive rats （ SHR ）. Methods： 60 SHR were randomized into modle group, Captopril treatment group, Niuhuang Jiangya pill treament group, Yangxue Qinnao treatment group 1 （ high dosage ）, group 2 （ medium dosage ）, group 3 （ low dosage ）. 10 WKY rats were regarded as normal group. After 8 weeks treatment, Ang Ⅱ and Aid were determined. Results：Yangxue Qingnao grain can reduce the level of the angiotensin Ⅱ not only in plasma but also in kidney. Conclusion：Yang xue Qingnao grain can reduce the blood pressure and protect the kidney,it＇s mechanism maybe is through it＇s renin-angiotensin system in kidney.