硝化速率法用于活性污泥活性测试的研究

Study on nitrification inhibition for measuring of activity of activated sludge

以城市污水厂回流污泥中的硝化细菌(氨氧化细菌和亚硝酸盐氧化细菌)为受试生物,HgCl2为标准毒性物质,通过对两组实验———氨氧化速率实验(NH4+-N→NO2--N)和亚硝酸盐氧化速率实验(NO2--N→NO3--N)的研究表明,氨氧化细菌对HgCl2的灵敏度(IC50=0.034 mmol/L)明显高于亚硝酸盐氧化细菌(IC50=0.20 mmol/L)。氨氧化速率法测试活性污泥活性时,使用NH4+-N或NOX--N指标需要120 min或更长的时间,但使用NO2--N指标仅需30 min就可完成测试,而且结果重现性要比NH4+-N和NOX--N好,其变异系数CV为5.9%。

Nitrosomonas and Nitrobacter, which were contained in activated sludge, were used for measuring of activity of activated sludge, and HgCl2 was chosen as the standard toxicant. Two methods ammonia oxidation and nitrite oxidation were studied respectively, and higher sensitivity was found with Nitrosornonas than with Nitrobacter （IC50=0. 034 mmol/L for Nitrosomonas, IC50 = 0.20 mmol/L for Nitrobacter）. When using ammonia oxidation for measuring activity of activated sludge, NH4^＋-N or NOx^--N could be determined, but more than 120 min were needed for good results. While NO2^--N was determined, good results could be obtained within 30 min with the smaller stand- ard deviation of the IC50 （CV= 5.9%）.