载脂蛋白H基因多态性与冠心病关系的研究

Relationship between apolipoprotein H Gene polymorphism and coronary heart disease

目的探讨载脂蛋白H(ApoH)外显子3、8基因多态性与冠心病(CHD)的关系。方法采用聚合酶链式反应结合限制性片段长度多态分析方法,分析了100例健康人及110例冠心病患者的ApoH外显子3、8基因型。结果CHD组外显3GG基因型的频率为81.8%,GA+AA基因型频率为11.8%,G等位基因频率为88%,A等位基因频率是12%,与对照组比较无差异,CHD组外显子8GG基因型频率为74.5%、GC基因型频率为25.5%,G等位基因频率为87%,C等位基因频率为13%,与对照组比较CHD组的GC基因型频率及C等位基因频率显著增高。结论ApoH外显子3基因多态性与CHD无相关性;8GC基因型及C等位基因与CHD有关。

Objective To investigate relationship between the polymorphism of apolipoprotein H（ApoH） exon3 and exon 8 polymorphismand coronary heart disease（CHD）. Methods Polymerase chain reaction and technique of restriction fragment length polymorphism were used to determine the genotypes of Aport exon 3 and exon8 in 110 patients with CHD and 100 health control group. Results The genotype GG of Aport exon 3 in the CHD group was 81.8% , GA ＋ AA genotype was only 11.8%, the frequency of G allele was 88%, while 12%in the A allele; there was no significant difference with the control groups The frequency of GG genotype in Aport exon 8 were 74.5 % ,GC genotype were25.5 %, the frequency of G allele was 87 % ; the frequency of GC genotype and C allele in CHD group were significant higher than that in control group. LDL level in CHD group was significant higher than that in control group（P〈0.05）. Conclusion The polymorphism of the Aport exon3 have no relationship with CHD but the GC genotyp of Aport exon3 and C allele have close relation with CHD and serum TG level.