摘要
为解决CHO-C28细胞在大规模培养中易增厚、大片脱落、不易维持以及HBsAg滴度低的问题,对细胞培养液pH值和新生牛血清(NCS)浓度进行调整。结果表明,CHO-C28细胞在2个月维持时间内未出现明显增厚和大片脱落,HBsAg表达量明显提高。该实验为大规模培养CHO-C28细胞提供了依据。
To resolve some questions on large scale CHO-C28 Cell culture such as incrassation, shedding and low HBsAg titer. A lot of experiments to change pH of medium and the consistence of newborn calf serum（NCS）, have been performed. The results showed that the phenomena mentioned above almost disappeared and the expression of HBsAg was obviously higher than before. This research is a founda- tion for the large-scale CHO-C28 Cell culture.
出处
《药物生物技术》
CAS
CSCD
2006年第2期112-114,共3页
Pharmaceutical Biotechnology
