摘要
研究发酵液中R-扁桃酸的离子交换分离工艺.采用711-OH型阴离子交换树脂从发酵液中分离R-扁桃酸时,最适动态吸附条件为上柱液pH值6.0～7.0、R-扁桃酸浓度70～80 mmol/L、苯乙酮酸浓度8～10 mmol/L和流速0.5 Bv/h,最适动态解吸条件为2% HCl为解吸液、解吸流速1.5 Bv/h.采用本工艺,发酵液中R-扁桃酸的总提取率为90.3%.
The purpose is to study a new method for the separation and purification of R-mandelic acid from ferment broth by ion exchange technology. While R mandelic acid separated from ferment broth at 711-OH anion exchange resin,the optimum dynamic adsorption condition was 6.0-7. 0 pH value, 70- 80 mmol/L R-mandelic acid concentration, 8 - 10 mmol/L phenylglyoxilic acid concentration and 0. 5 Bv/h velocity of flow. The optimum dynamic desorption condition was 2% HCl concentration and 0. ,5 Bv/h velocity of flow. 90.8% R-mandelic acid could be separated from ferment broth by this technicque.
出处
《药物生物技术》
CAS
CSCD
2006年第2期115-118,共4页
Pharmaceutical Biotechnology
基金
福建省自然科学基金(E0310019)
福建省科技厅重点项目基金(2003H023)
福建省计委基金(闽计2003-64号)资助
关键词
R-扁桃酸
发酵液
离子交换
711树脂
分离
R-mandelie acid, Ferment broth, Ion exchange, 711 anion exchange resin, Separation
