酶法合成6-氯嘌呤核苷

Enzymatic Synthesis of 6-Chloro-purine Riboside with Lactobacillus Helveticus

利用菌种Lactobacillus helveticus（ATCC 10697）所产生的N-脱氧核糖转移酶,以底物鸟苷和6-氯嘌呤为原料通过碱基交换合成6-氯嘌呤核苷,并优化了反应条件,25 m1的锥形瓶装有反应液10 ml,结果显示最佳反应条件如下：底物最适反应浓度为30 mmol/L（鸟苷）和10 mmol/L（6-氯嘌呤）,菌体添加量8%～10%（湿重）,反应温度40℃,0.1 mol/L磷酸缓冲液（pH值6.0）,摇床转速120 r/min,反应时间24 h,6-氯嘌呤核苷收率可达到51.6%,具有较好的应用前景.

The purpose is to improve the yield of 6-chloro-purinc ribosidc, which is one of intermediates for some new drugs, it was synthesized by using trans-N-dcoxyribosylase （EC 2.4.2.6 ） from Lactobacillus helvezicus （ATCC 10697） for catalyzing base group exchange from guanosine riboside and 6-chloro-purine in our lab. The optimal reaction conditions were studied in 25 ml shaking bottle with 10 ml so lution. The experiment showed that the optimum substrates concentration was 30 mmol/L （guanosine riboside）, 10 mmol/L （6-chlro-purine）. The conversion yield could reach 51.60% while in the reaction the substracts mixed with 8-10% wet whole cells and 0.1 mol/L phosphate buffer （pH 6.0）, and incubated at 40℃ for 2/1 h at the shaking speed of 120 r/min.