腺病毒介导的p53、GM-CSF和B7-1基因对Lewis肺癌的治疗作用

Therapeutic Effect of Adenovirus-Mediated Human Wild-Type p53,GM-CSF and B7-1 Genes on Lewis Lung Cancer in Mouse:An Experimental Study

目的:体内观察腺病毒介导的p53、GM-CSF和B7-1基因的抗肿瘤及协同化疗或放疗的作用。方法:建立C57小鼠的Lew is肺癌模型,瘤内注射不同剂量的上述三个基因重组的腺病毒,确定重组腺病毒治疗小鼠Lew is肺癌的有效剂量;与此同时观察有效剂量的重组腺病毒和放疗或化疗协同作用。结果:腺病毒介导的p53、GM-CSF和B7-1基因治疗小鼠Lew is肺癌的有效剂量为5×108pfu,该重组腺病毒与放疗或化疗联合应用可以显著提高小鼠Lew is肺癌对放疗或化疗的敏感性。结论:腺病毒介导的p53、GM-CSF和B7-1基因对小鼠Lew is肺癌具有良好的治疗作用;该重组腺病毒联合放疗或化疗可以显著提高小鼠Lew is肺癌对放疗或化疗的敏感性。

Objective：To observe in vivo anti-tumor effect of adenovirus-mediated human wild-type pS3,GM-CSF and B7-1 genes,their synergistic effects with chemotherapy or radiotherapy on Lewis lung cancer in mouse. Methods：Lewis lung cancer model in C57 mouse was established and the effective dose was determined by intratumorally injecting different dosages of Ad-p53 ,GM-CSF and B7-1 agent. The synergistic effects of recombinant adenovirus with chemotherapy or radiotherapy were observed at the same time. Results ：It was shown that 5×10^8 pfu of recombinant adenovirus had significant therapeutic effect on Lewis lung cancer. In addition ,when combined with chemotherapy or radiotherapy,the agent remarkably increased chemotherapy or radiotherapy sensitivity of cancer cells. Conclusion： Ad-p53, GM-CSF and B7-1 agent had satisfactorily therapeutic effect on Lewis lung cancer in C57 mouse. And the recombinant adenovirus significantly increased sensitivity of chemotherapy or radiotherapy by Lewis lung cancer cells.