妊娠肝内胆汁淤积症患者GSH-PX、LPO、SOD水平及临床意义

GSH-PX,LPO and SOD levels in pregnant women with intrahepatic cholestasis and their clinical significance

目的:探讨谷胱甘肽过氧化物酶(GSH-PX)、脂质过氧化物(LPO)、超氧化物歧化酶(SOD)与妊娠肝内胆汁淤积症(intrahepatic cholestasis of Pregnancy,ICP)的发病关系。方法:采用二硝基苯甲酸直接法、硫代巴比妥酸荧光测定法及速率法测定35例ICP患者和20例正常孕妇及两组孕妇新生儿脐血全血GSH-PX、血浆LPO、SOD水平,同时测定样本肝功能指标(TB IL、TBA)进行相关性分析。结果:ICP组GSH-PX水平较对照组显著降低,差异显著(P<0.01)。ICP组LPO、SOD水平较对照组明显升高,差异性显著(P<0.01)。ICP患者新生儿脐血GSH-PX、LPO、SOD水平明显高于正常对照孕妇脐血水平,差异性显著(P<0.01)。ICP患者GSH-PX、LPO、SOD水平与其TB IL、TBA水平呈正相关(P<0.05或P<0.01)。结论:ICP患者GSH-PX、LPO、SOD含量明显发生异常变化,提示其在ICP发病及病理过程中具有重要作用。

Objective： To explore the effect of glutathione peroxidase （ GSH - PX）, lipid peroxidation （ LPO ） and superoxide dismutase （SOD） on intrahepatic cholestasis of pregnancy （ICP） . Methods： 35 cases of ICP, 20 cases of healthy pregnant and their neonatal cord blood were chosen to detect GSH - PX, LPO and SOD levels by. 5.5 - dithionbis （2 - nitrobenzoic acid）, thiobarbiuric acid flurophotometry and reaction rate assay, and to determine TBIL and TBA to conduct correlative analysis. Results ： The level of GSH - PX in the ICP group was significantly lower than that in control group （P〈0.01 ） . LPO and SOD levels in ICP group were significantly higher than those in control group （ P〈0.01 ） . GSH - PX, LPO and SOD levels in ICP were positively correlated with TBIL and TBA. Conclusion： Significantly abnormal content change of GSH - PX, LPO and SOD might play an important role in the course and pathogenic mechanism of ICP.