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超大容量非免疫人源性Fab抗体库的构建 被引量：4

The construction of super-large human antiboby library.
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摘要 目的构建超大容量非免疫人源性Fab抗体库。方法通过RT-PCR从208名不同人的外周血淋巴细胞中扩增出人IgG重链Fd段和κ、λ两轻链基因片段,将其克隆入噬粒pComb3中,构建人源性Fab段噬菌体抗体库。结果成功地构建了库容量为2.6×1012的人源性Fab段噬菌体抗体库。结论新的噬菌体抗体库对筛选不同抗原位点的抗体,提供有用的资源。 Objective To construct a human Fab phage antibody library.Methods Fd heavy chain gene and κ、λ light chains gene of IgG obtained by RT- PCR from 208 samples of different human lymphocytes were cloned into p Comb3 vector and the human Fab phage anti- body library was constructed. Results A human Fab phage antibody library with 2.6 × 10^12 capability was successfully constructed. Conclusion This new phage antibody library is set to become a valuable source of antibodies to many different targets.
作者 李晓琳 侯宗柳 刘建生 孟明耀 马进 王萍
机构地区 中国医学科学院中国协和医科大学医学生物学研究所
出处 《云南医药》 CAS 2006年第2期94-97,共4页 Medicine and Pharmacy of Yunnan
关键词 噬菌体抗体库 人源性Fab抗体 Phage antibody library Human Fab fragment antibody
分类号 R33 [医药卫生—人体生理学]
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参考文献8

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同被引文献40

  • 1马小兵,畅继武,王海涛,王馨,马腾骧.人天然Fab段噬菌体抗体库的构建及初步鉴定[J].卫生研究,2006,35(2):206-209. 被引量：5
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  • 3Matthias Paschke. Phage display systems and their applications [ J ]. Appl Microbiol Biotechnol, 2006; 70( 1 ) : 2-11.
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  • 6Hoogenboom H R. Designing and optimizing library selection strategies for generating high-affinity antibodies [ J ]. Trends Biotechol, 1997 ; 15 (2) : 62- 70.
  • 7Sheets M D, Amersdorfer P, Finnem R et al. Efficient construction of large non-immune phage antibody library: the production of high affinity human single-chain antibodies to protein antigens[J]. Proc Natl Acad Sci USA, 1998;95(11 ) :6157-6162.
  • 8Sblattero D, Bradbury A. Exploiting recombination in single bacteria to make large phage antibody libraries[J]. Nat Biotechnol,2000; 18( 1 ) :74- 80.
  • 9de Haard H J, van Neer N, Reurs A et al. A large nonimmunized human Fab fragment phage library that permits rapid isolation and kinetic analysis of high affinity antibodies[J]. J Biol Chem, 1999;274(26):18218- 18230.
  • 10Titus Kretaschmar, Thomas von Ruden. Antibody discovery: phage display [J]. Current Opinion in Biotechnology,2002; 13(6) :598-602.

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云南医药
2006年 第2期

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