应用基因芯片研究环孢素对大鼠肝脏基因表达的影响

Study of Effect of Cydosporin A on Gene Differential Expression in Livers by cDNA Microarray in Rats

目的研究环孢素对大鼠肝脏基因表达谱的影响。方法大鼠予以环孢素灌胃1次,摘取肝脏提取总RNA。用Cy3和Cy5 两种荧光物质分别标记阴性对照组和环孢素组肝组织cDNA,制备成探针与表达谱芯片进行杂交,获得的荧光信号图象用计算机扫描后进行数据分析,计算每点的Cy5和Cy3信号强度比值。结果在被检测的4096个基因中,环孢素组差异表达基因50个,占芯片基因总数 1．22％,其中表达上调的基因17个,表达下调的基因33个。结论环孢素的免疫抑制、抗肿瘤作用以及与其他药物的相互作用可能与一系列代谢相关基因的表达变化有关。

Objective To study the effects of cyclosporin A （CsA） on genes expression in rat liver. Methods The rats were sacrificed 24 hours after being orally treated once with cycolsporin A and the livers were taken out to extract total RNA. The cDNA derived from negative control group and CsA group livers were labeled with Cy3 - dCTP and Cy5 - dCTP respectively. These probes were hybridized with cDNA microarray. The acquired image was scanned and analyzed by GenePix Pro 3.0 software. The intensity ratio of Cy5 to Cy3 about each spot was calculated. Results Among 4 096 genes, 50 genes exhibited differential expression in CsA group ,of which, 17 genes were up - regulated and 33 genes down - regulated. Conclusions The immuno - inhibitive effect, anti - cancer effects and interactions between CsA and other drugs may be related with the changes in the expressions of some associated genes.