血小板反应素mRNA表达与甲状腺乳头状癌血管生成、转移的关系

Expression of TSP mRNA and its relation with angiogenesis and lymph node metastasis in papillary thyroid carcinoma

目的研究甲状腺乳头状癌组织中血小板反应素(TSP)表达与微血管密度(MVD)及淋巴结转移之间的关系。方法对40例甲状腺乳头状癌手术标本和10例癌旁正常组织标本采用逆转录-多聚酶链反应(RT-PCR)检测TSP1和TSP2mRNA表达,并采用CD105单克隆抗体标记免疫组化法检测微血管密度。结果正常组织TSP1mRNA阳性率为80.0%,TSP2mRNA阳性率为90.0%;癌组织TSP1mRNA阳性率为45.0%,TSP2mRNA阳性率为47.5%,二者表达组MVD(25.4±11.7vs23.9±16.4)低于不表达组(43.6±10.4vs45.1±11.9),MVD差异具有统计学意义(t值分别为2.36和2.64,P均<0.05),二者同时表达者MVD更低于同时不表达者(P<0.01),TSP1mRNA和TSP2mRNA阳性表达率在发生淋巴结转移的病例显著低于未发生转移的病例(χ2=9.42,P<0.01)。结论甲状腺乳头状癌组织TSPmRNA表达可降低MVD和抑制淋巴结转移。

Objective To study the expression of thrombospondin（TSP） mRNA and its relationship with angiogenesis and lymph node metastasis in papillary thyroid carcinoma（PTC）. Methods RT-PCT was utilized to detect the expression of TSP1 and TSP2 mRNA in 40 cases of PTC and 10 cases of normal tissue surrounding carcinoma. Immunohistochemical stain was performed to detect microvessel density（MVD） whose endothelia were marked by anti-CD105 McAb. Results The positive- expression rate of TSP1 mRNA,TSP2 mRNA was 80.0%, 90.0% respectively in normal tissue surrounding carcinoma. The positive-expression rate of TSPI mRNA was 45.0 %. The positive-expression rate of TSP2 mRNA was 47.5%. The MVD value of PTC cases with TSP1 and TSP2 mRNA positive expression was significantly lower than that of cases without TSP1 and TSP2 mRNA expression（P〈 0.05）. The MVD value of the cases with both TSP1 and TSP2 mRNA positive was also significantly lower than that of the one with neither TSP1 nor TSP2 mRNA expression（P〈0.01）. The expression rates of TSP1 and TSP2 were higher in PTC cases with lymph node metastasis（χ^2 =9.42,P〈0.01）. Conclusion The expression of TSP mRNA may serve as a role in the decrease of MVD and prohibition of lymph node metastasis.