摘要
目的探讨凯西莱(硫普罗宁)防治大鼠免疫性肝纤维化的效果与相关机制。方法48只SD大鼠,雌雄各半,体重无明显差异,随机配对分为对照组、模型组、凯西莱组。模型组、凯西莱组腹腔内注射猪血清诱导大鼠免疫性肝纤维化,0.5ml/只,1次/d,共12周。与之同时,凯西莱组经口灌胃给予凯西莱,0.5ml.只-1.d-1(含硫普罗宁50mg.kg-1.d-1),共16周。而对照组、模型组则经口灌胃给予生理盐水,0.5ml.只-1.d-1,共16周。第12周,停止腹腔内注射猪血清,各组随机处死一半大鼠。第16周,处死余下的全部大鼠。检测血清丙氨酸转氨酶(ALT)、谷氨酰转肽酶(-γGT)、清蛋白(ALB),及肝组织中丙二醛(MDA)、还原型谷胱苷肽(GSH)、羟脯氨酸(HYP)等含量。HE染色检查肝组织病理变化。结果(1)在第12周,凯西莱组与模型组比较,血清ALT、-γGT降低,清蛋白升高,肝脏中MDA、HYP降低,GSH升高。肝纤维化程度减轻。(2)凯西莱组在第16周与其第12周比较,肝脏的纤维化程度和HYP含量无明显变化,血清ALT、γ-GT降低,清蛋白升高,肝脏中MDA降低,GSH升高。结论凯西莱防治大鼠免疫性肝纤维化有效。保护肝细胞,降低脂质过氧化反应是其抗肝纤维化的机制之一。
Objective To investigate the efficacy and mechanism of tiopronin against the hepatic fibrosis induced by the porcine serum in rats. Methods Fourty-eight SD rats were randomly divided into the control group, the model group and tiopronin group. Female and male rats were equal in numbers, and body weight was not significantly different among the groups. The model group and the tiopronin group were dealt with the porcine serum via the intraperitoneal injection, 0. 5ml per rat, twice per week, 12 weeks in all, respectively. However, the control group and the model group, who were managed as to the tiopronin group except the drug tiopronin [0.5ml per rat (50mg · kg^-1· d^-1 ), once a day, 16 weeks in all], were dealt with the normal saline via intragastric administration. In 12 weeks, the porcine serum injection stopped and half of rats in each group were killed for examinations, respectively. In 16 weeks, the rest were killed. To examine the concentrations of alanine transaminase(ALT), glutamyl transpeptidase(γ-GT), albumin(ALB) in serum as well as malondialdehyde(MDA), reduced glutathione (GSH), hydroxyproline (HYP) in liver tissue. In addition, the level of the hepatic fibrosis was assessed by a pathologist. Result (1) Compared the tiopronin group in 12 weeks with the model group in 12 weeks, ALT, y-GT decreased, but ALB increased. However, in liver tissue, MDA, HYP decreased, GSH increased, and the level of the hepatic fibrosis decreased. (2) Compared tiopronin group between 16 weeks and 12 weeks, ALT, γ-GT decreased, ALB increased, MDA decreased, GSH increased. But the significant change in the level of hepatic fibrosis and the concentrations of HYP could not bee seen. Conclusion Tiopronin is effective to the prevention and treatment on the hepatic fibrosis, one of its mechanisms relates to protect the liver cell and alleviate lipid peroxidation.
出处
《重庆医学》
CAS
CSCD
2006年第8期697-698,702,共3页
Chongqing medicine