化疗对结肠癌Th1和Th2类细胞因子基因表达的影响及意义

Influence and significance of chemotherapy to the gene expression of Th1 and Th2 cytokines in colon carcinoma

目的探讨结肠癌患者体内Th1和Th2类细胞因子的基因表达水平及化疗对其表达的影响。方法收集67例结肠癌患者、35例正常人和30例结肠息肉患者血清。采用RT-PCR技术,检测3组及结肠癌组化疗后外周血单个核细胞(PBMC)Th1和Th2类细胞因子mRNA表达水平。结果正常对照及结肠息肉组Th1和Th2类细胞因子基因表达呈阴性或基本不表达。结肠癌组IL-4、IL-6、IL-10mRNA表达水平显著高于正常对照及结肠息肉组(P<0.05),且与结肠癌的分化程度及临床分期相关(P<0.05);IFN-γ无阳性表达(0/67),IL-2则仅有3例阳性表达,其表达与结肠癌的分化程度及临床分期无相关性(P>0.05)。化疗后Th1类细胞因子表达增强,Th2类细胞因子的表达则减弱(P<0.05)。结论结肠癌患者Th1和Th2类细胞因子表达失衡,细胞免疫受抑,化疗可使结肠癌患者Th2类细胞因子的强势表达向Th1类逆转。

Objective： To observe the influence of chemotherapy to the switch of Th1/Th2 cytokines in colon neoplasms. Methods：The gene expression of Th1/Th2 cytokines was detected by RT-PCR in 3B normal controls ,30 cases with colic polyposis and 67 patients with colon neoplasms before and after chemotherapy. Results： Th1 and Th2 cytokines were negative in peripheral blood from those with colic polyposis and the normal controls. The mRNA expression of IL-4, IL-6, IL-10 in PBMC from patients with colon carcinoma was obviously higher than those from colic polyposis and the normal controls（P〈0.05）, and collected with the patients＇clinical stages and differential degrees（P〈0. 05）. IFN-γ expression was negative in all patients （0/67） ,and the positive expression of IL-2 was only 3/67 ,and no relevance with the patients ＇clinical stages and differential degrees. After chemotherapy, the expression of Th1 and Th2 cytokines was changed obviously, which reflected by a significant enhancement in IFN y and IL-2 expression, parallelling a substantial decrease in IL-4, IL-6, IL 10. Conclusion： The expression of Th1 and Th2 cytokines is imbalance in PBMC from patients with colon carcinoma;Chemotherapy can convert the predominant state of Th2 make it incline to the the normal state.