提高狗蔷薇离体培养植株再生频率

Improving of in vitro Plant Regeneration Frequency of Rosa canina (Rosaceae) Inermis

以狗蔷薇(RosacaninaInermis)为材料,以MS为基本培养基,通过对不同植物生长调节剂的组合,大幅度提高了狗蔷薇离体培养植株再生频率。结果表明,2.0mgL6BA+0.3mgL2,4D的组合较为适宜,其不定芽再生率为87%,增殖率为3.0;而CPPU和2,4D的适宜组合为1.5mgL+0.3mgL,其不定芽再生率高达93%,增殖率为5.0。同时,研究结果显示,以MS+40gL蔗糖+6.0gL琼脂粉+3.5mgLAgNO3+1.5mgLCPPU+0.1mgL2,4D+0.05mgLGA3作增殖培养基效果最好,不定芽诱导率为89%,增殖率为5.5;利于生根的培养基为14MS+20gL蔗糖+3.5gL琼脂+0.3%活性碳+0.1mgLIBA+0.1mgLNAA,生根率为91%。

An efficient regeneration system of Rosa canina Inermis was developed in this study. The results showed that the regeneration frequency of adventitious buds was 87% and the multiplication ratio was 3.0 when supplemented with 2.0 mg/L 6-BA and 0.3 mg/L 2,4 - D on Murashige and Skoog （MS） medium, while the regeneration ratio was 93% and the multiplication ratio was 5.0 when the MS medium contained 1.5 mg/L CPPU and 0.2 mg/L 2,4 - D. Moreover, the result also indicated that CPPU was more effective in the induction of adventitious buds. The optimal medium for the multiplication of shoots was MS ＋ 40 g/L sucrose ＋ 6.0 g/L agar ＋ 3.5 mg/L AgNO3 ＋ 1.5 mg/L CPPU ＋ 0.1 mg/L 2,4 - D ＋ 0.05 mg/ L GA3, in which the multiplication ratio was 5.5 and the adventitious bud induction frequency was 89%. The best medium for rooting and growing was 1/4MS ＋ 20 g/L sucrose ＋ 3.5 g/L agar ＋ 0.3% activated carbon ＋ 0.1 mg/L IBA ＋ 0.1 mg/ LNAA. The rooting frequency was 91%.