鼠疫胶体金免疫层析试纸条在新疆、内蒙的现场应用评价

The effectiveness evaluation of Gold Immunochromatographic Assay in testing plague field samples of XinJiang and NeiMeng province

目的:评价检测鼠疫抗原及抗体胶体金免疫层析试纸条(GICA及RGICA)在新疆和内蒙现场材料的检测效果。方法:1.分别对1404份血清标本(新疆1304份,内蒙100份)采用GICA、IHA及ELISA进行检测;2.分别对334份鼠脏器材料(新疆224份,内蒙100份)采用RGICA、RIHA及F1-ELISA进行检测。结果:1.在1304份新疆血清标本的检测中,GICAI、HA及ELISA三种方法的符合率高度一致,而GICA的敏感性比IHA与ELISA分别高32%和3.1%;在100份内蒙血清标本的检测中,GICA与IHA的检测结果完全一致。2.在224份新疆鼠类脏器标本检测中,RGICA与RIHA、F1-ELISA及检菌三种方法的符合率高度一致,而RGICA的敏感性比RIHA与F1-ELISA分别高22.2%和10%;在100份内蒙鼠类脏器标本的检测中,RGICA与检菌结果完全一致,而与RIHA高度一致,RGICA比RIHA的敏感性高13%。结论:研制成功的检测鼠疫的GICA及RGICA在现场材料的检测中特异性强、灵敏度高、简便快速,有较大的推广应用价值。

Objective： To evaluate the effectiveness of Gold Immunochromatographic Assay in testing plague field samples of Xinjiang and NeiMeng province. Methods： 1. We tested 1404 serum samples （1304 samples of XinJiang and 100 samples of NeiMeng ） with GICA , IHA and ELISA. 2. We tested 334 mice viscera samples （224 samples of Xinjiang and 100 samples of NeiMeng ） with RGICA ,RIHA and F1-ELISA. Results： 1. There are high consistence among GICA, IHA and ELISA in testing 1304 Xinjiang serum samples. Moreover, GICA is more sensitive than IHA and ELISA by 32 % and 3.1% respectively. There are the same result in testing 100 samples of NeiMeng with GICA and IHA. 2. There is high consistence among RGICA, RIHA and F1-ELISA in testing 224 viscera samples of Xinjiang, Moreover, RGICA is more sensitive than RIHA and F1-ELISA by 22.2% and 10% respectively. There is the same result in testing 100 samples of NeiMeng with RGICA and germ culture. RGICA is high consistence with RIHA, and more sensitive than RIHA by 13%. Conclusions： GICA and RGICA are sensitive, specific, simple and rapid assays for detecting Yersinia pestis in field samples. So GICA and RGICA have good prospect in testing field samples of plague.