7016株临床分离病原菌耐药性分析

Surveillance of Antimicrobial Resistance of 7 016 Strain Pathogens

目的了解临床常见细菌的分布及对各种抗菌药物耐药性现状。方法对住院患者分离出的主要病原菌用K-B法进行药敏试验,用WHONET5.3软件进行耐药统计分析。结果共分离病原菌7 016株,革兰阳性菌2 250株,以葡萄球菌属为主,其中耐苯唑西林株分别占金黄色葡萄球菌和凝固酶阴性葡萄球菌的62.1%和76.0%;检测中未发现耐万古霉素葡萄球菌;革兰阴性菌4 766株,主要由铜绿假单胞菌、大肠埃希菌、肺炎克雷伯菌和不动杆菌构成;铜绿假单胞菌对亚胺培南的耐药率逐年升高。结论临床分离菌中凝固酶阴性葡萄球菌及耐苯唑西林葡萄球菌比例逐年升高,铜绿假单胞菌对亚胺培南的耐药问题较严重。

OBJECTIVE To investigate the antimicrobial resistance of nosocomial infection pathogens and to provide the reference for the clinical treatment and infection control in hospital. METHODS Bacteria were isolated from patients in our hospital from Jan 2000 to Dec 2004 and animicrobial susceptibility was tested by disc diffusion method（K-B method）. RESULTS A total of 7016 strain pathogens were isolated,among them 2250 strains were Gram-positive cocci, The most common pathogens of them were Staphylococcus. Meticillin resistant strains of S. aureus and coagulase negative Staphylococcus（CNS） accounted for 62.1% and 76.0%, respectively. There were 4766 strains of Gram-negative bacilli. The most common pathogens of them were Pseudomonas aeruginosa, Klebsiella pneurnoniae, Escherichia coli, Acinetobacter and Enterobacter cloacae. The most common ESBLs producing strains were K. pneumoniae and E. coli. In our data, no vancomycin resistant Staphlococcus was isolated. The imipenem resistant P. aeruginosa was growing by year. CONCLUSIONS Coagulase negative Staphylococcus and meticillin resistant Staphylococcus are growing, imipenem resistance of P. aeruginosa is a serious problem.