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大鼠SR-BI胞外域与ApoA-I蛋白相互作用的确认 被引量：1

Verification the Interaction of Apolipoprotein AI and the Extracellular Domain of Scavenger Receptors Class B Type I
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摘要 先以含全长SR-BIcDNA序列的重组质粒pMD18-T-rS为摸板进行PCR反应扩增SR-BI得到胞外域cDNA片段,经测序证明正确后,定向克隆到酵母双杂交表达载体,然后与pGBKT7-ApoA-I质粒共转化酵母细胞,通过报告基因及酵母交配试验确认了SR-BI的胞外域部分和ApoA-I之间的确存,观察到ApoA-I与SR-BI胞外域间的相互作用力比与全长的SR-BI间的相互作用力提高了10%。 In this paper, the DNA fragment rSE was amplified by PCR using the recombinant plasmids pMD18- T-rS （containing the hole lines of cDNA of SR-BI） as template. Then the PCR product was directionally cloned into the yeast two hybrid express vector pGADT7 after it was confirmed with sequencing. The recombined vector （named pGADTT-rSE） and pGBKTT-ApoA-Ⅰ vector was cotransformed in yeast strain AH109. We identified the interaction of APOA-Ⅰ and rSE by using report genes and yeast mating, At last, we obsevered that the interaction of APOA-Ⅰ and rSE is about 10% more than that of ApoA-Ⅰ and SR-BI of rat.
作者 李兰松 李维琪 信学雷 曹旭
机构地区 中国科学院新疆理化技术研究所
出处 《微生物学通报》 CAS CSCD 北大核心 2006年第2期68-73,共6页 Microbiology China
基金 中国科学院"百人计划"项目资助 2001年"西部之光"项目资助
关键词 载脂蛋白AI(ApoA-Ⅰ) B族I型清道夫受体(SR-BI) SR-BI胞外域 基因克隆 酵母双杂交 Apolipoprotein AI （ ApoAⅠ）, Scavenger receptor class B type Ⅰ （ SR-BI）, Extracellular domain of SR-BI, Gene clone, Yeast two hybrid
分类号 R341 [医药卫生—基础医学]
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参考文献10

  • 1Acton S,Rigotti A,Landschulz K T,et al.Science,1996,271:518～520.
  • 2Out R,Hoekstra M.J Lipid Res,2004,45:2088～2095.
  • 3Williams D L,de La Llera-Moya M,Thuahnai S T,et al.J Biol Chem,2000,275 (25):18897 ～18904.
  • 4Cao G,Garcia C K,Wyne K L,et al.J Biol Chem,1997,272 (52):33068 ～33076.
  • 5信学雷,陈志慧,李维琪,刘云英,麦迪娜,张云峰.用酵母双杂交系统研究载脂蛋白AI(apoAI)和清道夫受体BI(SR-BI)间的相互作用[J].中国生物工程杂志,2005,25(5):80-84. 被引量：3
  • 6信学雷,李兰松,阿吉.艾克拜尔.艾萨,茹仙古丽.莎吾尔,李维琪,阿力木江.阿布地热力木.2种维药复方对apo-AI和SR-BI之间相互作用的影响[J].中国中药杂志,2005,30(13):1041-1043. 被引量：1
  • 7Clontech MATCHMAKER GAL4 two-hybrid system3.1999.6.
  • 8Williams D L,Temel R E,Cormelly M A.Endocr Res,2000,26 (4):639 ～651.
  • 9Connelly M A,de la Llera-Moya M,Monzo P,et al.Biochemistry,2001,40 (17):5249 ～5259.
  • 10Silver D L,Tall A R.Curr Opin Lipidol.2001,12 (5):497～504.

二级参考文献27

  • 1萨姆布鲁克J 拉塞尔DW 黄培堂 王嘉玺 朱厚础 译.分子克隆实验指南(第3版)[M].北京:科学出版社,2002.2-140.
  • 2Koji M, Yoshinaru W, Takaaki N. Effects of glucose and insuslin on rat apoliporoteinA-I gene expression. J Bio Chem, 1998, 273(30) : 18959 - 18965.
  • 3Ponsin G T, Pulcini J T, Sparrow A M, et al. High density lipoprotein interconversions in rat and man as assessed with a novel nontransferable apolipopeptide. J Biol Chem, 1993, 268 : 3114 -3119.
  • 4Gao G, Garcia C K, Wyne K L, et al. Structure and localization of the human gene encoding SR-BI/CLA-1 : evidence for transcriptional control by steroidogenic factor 1. J Biol Chem,1997, 272 : 33068 - 33076.
  • 5Trigatti K B, Rayburn H, Vinals M, et al. Influence of the highdensity lipoprotein receptor SR-BI on reproductive and cardiovascular pathophysiology. Proc Nail Acad Sci USA, 1999,96 : 9322 - 9327.
  • 6Temel R E, Walzem R L, Banka C L, et al. Apolipoprotein A-I is necessary for the in Vivo formation of high-density lipoprotein competent for scavenger receptor BI-mediated cholesteryl esterselective uptake. J Biol Chem, 2002,277(29): 26565 - 26572.
  • 7Liu T, Krieger M, Kan H Y, et al. The effects of mutations in helices 4 and 6 of APOA-I on scavenger receptor class B type I(SR-BI)-mediated cholesterol efflux suggest that formation of a productive complex between reconstituted high density lipoprotein and SR-BI is required for efficient lipid transport. J Biol Chem,2002, 277 (24): 21576 - 21584.
  • 8Greene D J, Skeggs J W, Morton R E. Elevated triglyceridec content diminishes the capacity of high density lipoprotein to deliver cholesteryl esters via the scavenger receptor class B type I (SRBI). J Biol Chem, 2001, 276(7): 4804-4811.
  • 9Vials M, Xu S, Vasile E, et al. Identification of the N-linked glycosylation sites on the high density lipoprotein (HDL) receptor SR-BI and assessment of their effects on HDL binding and selective lipid uptake. J Biol Chem, 2003,278(7) : 5325 - 5332.
  • 10Connelly M A, de la Llera-Moya M, Peng Y, et al. Separation of lipid transport functions by mutations in the extracellular domain of scavenger receptor class B type I. J Biol Chem, 2003,278 (28) :25773 - 25782.

共引文献2

同被引文献23

  • 1李莉,张锦,彭阳,刘聪,李强,周一军,孟馨.代谢综合征小鼠肝脏改变及肝脏清道夫受体B1的表达[J].中国老年学杂志,2004,24(12):1168-1171. 被引量：4
  • 2胡海,所广军,杨士勇,蔡劬,姜志宏,蔡杏兴,袁作彪,蒋兆彦,张圣道,韩天权.胆固醇结石病人肝脏SRBI及其转录调节因子LRH-1基因表达的研究[J].外科理论与实践,2006,11(4):333-336. 被引量：14
  • 3Pahofer KG. Beyond I.DL - cholesterol : HDL - cholesterol as a target for atherosclerosis prevention [J]. Exp Clin End Diab, 2005, 113(8) :414 -417.
  • 4Mineo C, Yuhanna IS, QuonMJ, et al. High density lipoprotein - induced endothelial nitric - oxide synthase activation is mediated by Akt andMAP kinases[J]. J Biol Chem,2003,278(11) :9142 - 9149.
  • 5Assanasen C, Mineo C, Seetharam D, et a l. Cholesterol binding, efflux,and a PDZ- interacting domain of scavenger receptor- BI mediate HDL- initiated signaling[J]. J Clin Invest,2005,115(4) : 969 - 977.
  • 6Voisset C, Callens N, Blanchard E, et al. High density lipoproteins facilitate hepatitis C virus entry through the scavenger receptor class B type Ⅰ[J]. J Biol Chem,2005,280(9):7793 - 7799.
  • 7Grove J, Huby T,Stamataki Z,et al. Scavenger receptor B Ⅰ and B Ⅱ expression levels modulate hepatitis C virus infectivity[J]. J Viro,2007,81(7) :3162 - 3169.
  • 8Philips JA, Rubin EJ,Perrimon N. Drosophila RNAi screen reveals CD36 family member required for mycobacterial infection[J]. Science, 2005,309(5738) : 1251 - 1253.
  • 9Ohgami N, Miyazaki A, Sakai M, et al. Advanced glycation end products(AGE)inhibit scavenger receptor class B type I- mediated reverse cholesterol transport: A new crossroad of AGE to cholesterol metabolism[J]. J Atheroscler Thromb, 2003,10 ( 1 ) : 1 - 6.
  • 10Connelly MA, Williams DL. Scavenger receptor BI: A scavenger receptor with a mission to transport high density lipoprotein lipids[J]. Curr Opin Lipido, 2004,15 : 287 - 295.

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微生物学通报
2006年 第2期

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