人乳头瘤病毒芯片寡核苷酸探针的研究

Study of Type-specific Oligonucleotide Probes for Microarray Detection of Human Papillomavirus

高危型人乳头瘤病毒(Humanpapillomavirus,HPV)是宫颈癌的主要致病因子。利用Arraydesigner2·0和BLAST等生物学软件对10种型别的人乳头瘤病毒全基因组序列进行分析,设计高特异性、熔解温度(Tm)和GC含量相近的60merHPV型特异性寡核苷酸探针,用于HPV检测芯片的制备,并对其中四型最常见HPV病毒(HPV6,11,16,18探针的有效性进行初步验证,结果表明设计所得的探针型特异性好,可以应用于HPV的检测与分型。

High-risk human papillomavirus （HPV） types are major cauls of cervical cancer. By using bioinformatics softwares such as BLAST, Arraydesigner2.0 to analyze the whole genome sequence of 10 prevalent HPV types and design type-specific oligonucleotide microarray probes of high specificity, identical length and similar melting temperature （Tm） . These type-specific oligonucleotide probes were used to prepare microarray for HPV detection. And the validity of four HPV types （HPV 6, 11, 16, 18 ） probes were validated preliminarily, showing that most of the type specific oligonucleotide probes can be effectively applied to the HPV detection and typing microarray.