热带假丝酵母正烷烃代谢研究及代谢工程方法进展

Advance in Metabolism of n-Alkane and Metabolic Engineering of Candida tropicalis

正烷烃在热带假丝酵母(Candidatropicalis)细胞内的代谢过程主要包括ω-氧化和β-氧化两个反应途径,前者促使脂肪酸的生成,后者则涉及脂肪酸的降解,二者均由一系列的酶催化完成。本文综述了烷烃代谢过程的机理,以及各种相关酶及其基因的研究状况。在此基础上,进一步对与C·tropicalis有关的分子遗传学方法,以及代谢工程方面在近年来的研究进展进行了介绍。

Metabolism of n-alkane mainly includes w-oxidation pathway and β-oxidation pathway in Candida tropicalis, which are both catalyzed by a series of enzymes. The transformation from n-alkane to fatty acid is accomplished via w-oxidation pathway, and the degradation of fatty acid is involved in β-oxidation pathway. The mechanism on metabolism of n-alkane and studies on related enzymes are summarized in the review. Furthermore, some molecular genetics and metabolic engineering techniques related to Candida tropicalis developed in recent years are introduced.