基于去保护机理的新型H_2O_2荧光探针的制备及应用

Synthesis and Application of Novel H_2O_2 Fluorescent Probe Based on the Deprotection Mechanism

合成的3',6'-对戊酰硼荧光素是一种基于去保护机理的高灵敏H2O2荧光增强化合物,能灵敏地检测出样品中H2O2的含量.用IR,1HNMR对该探针的结构进行了表征,并且讨论了反应时间、溶剂、碱对探针合成的影响.研究了探针的最佳响应时间,结果表明检测过程中反应的最佳时间为3min.该探针在2.3×10-12～2.0×10-8mol/LH2O2溶液范围内呈荧光增强趋势,检测下限为2.3×10-12mol/L.此探针重现性良好,在血清中检测H2O2的回收率在93.9%～102.2%之间,结果令人满意,具有一定的实用价值.

The compound, 3＇,6＇-bis（tetramethylethylenedioxyboryl）fluorane, synthesized in this experiment is a kind of H2O2 fluorescence-strengthened reagent, which depends on deprotection mechanism and can examine the H2O2 concentration sensitively. The structure of the compound was confirmed by the IR and ^1H NMR spectra. The influences of reaction time, solvent and base on the probe synthesis were investigated in detail. The response time of the fluorescent probe was also studied and as a result, the optimal time for the H202 detection is 3 min. The fluorescence intensity of the probe was gradually increased with the increase of H202 concentration in the range of 2.3-10^-12～2.0×10^-8 mol/L, the detection limit of H2O2 was 2.3×10^-12 mol/L, and the recovery rates in the blood serum were in the range of 93.9%～102.2%.