肝炎平对肝纤维化肝组织中去甲肾上腺素和多巴胺的影响

The effect of Ganyanping on Norepinephrine and Dopamine in liver tissue of fibrosis

目的:探讨肝炎平在四氯化碳(CCl4)诱导的肝纤维化模型中对肝脏中去甲肾上腺素(NE)和多巴胺(DA)的影响及其抗肝纤维化的可能机制。方法:CCl4诱导大鼠慢性肝纤维化模型。随机分为正常组、肝纤维化模型组和肝炎平治疗组。用高效液相色谱-电化学(HPLC-ECD)法检测肝组织中NE和DA水平。结果:肝炎平组和模型组大鼠肝组织内NE含量明显低于正常组(P<0·01),且模型组NE含量低于肝炎平组(P<0·05);而DA在3组中的含量差异无显著性意义(P>0·05)。结论:肝炎平可增加肝纤维化肝组织中NE的含量,可能主要通过调节肝纤维化大鼠肝脏中的NE含量而达到抗肝纤维化的作用。

Objective： To investigate the effect fo Ganyanping （GYP） on Norepinephrine （NE） and Dopamine （DA） and the possible mechanism resisting hepatic fibrosis induced by carbon tetrachloride （CCI4）. Methods： The 40 rats were randomly diveded into control group, model group and GYP-treated group. The NE and DA concentrations in liver tissue were determined with high performanec liquid chromatography-electrochemical detector （HPLC-ECD）. Results： The levels of NE in model and GYP-treated group were obviously less than in control group （P〈0.01）, and NE in model group was less than in GYP-treated group （P〈0.05） ; while DA was similar in the three groups （P〉0.05）. Conclusion： GYP could increase the NE concentrations in liver tissue of fibrosis, and achieved to inhibit the hepatic fibrosis mainly by regulating the levels of NE in rats with hepatic fibrosis.