知柏地黄丸对肾上腺皮质激素型肾阴虚幼龄大鼠血浆CORT、ACTH、CRH及肾上腺指数和组织学结构的影响

Effects of ZhiBaiDiHuang Pill on Levels of CORT,ACTH,CRH in Blood and Index of Adrenal and Tissue Structure of Adrenal in Young Rats Model of Kidney-Yin Defiency of Adrenal Cortex Hormone

目的:探讨知柏地黄丸对肾阴虚幼龄大鼠血CORT、ACTH、CRH及肾上腺指数和肾上腺组织学结构的影响。方法:用氢化可的松每日腹腔注射法造成肾阴虚模型。结果:与空白组比较,模型组cAMP含量无变化,cGMP含量明显降低,cAMP/cGMP含量明显升高,说明造模成功。与模型组比较,知柏地黄丸高剂量组可明显提高血浆CORT、ACTH、CRH及肾上腺指数。其疗效明显优于六味地黄丸组。知柏地黄丸低剂量组与六味地黄丸组比较,治疗效果无显著差异。镜下观察可见,模型组肾上腺各层明显变薄,皮质层萎缩尤为明显,部分细胞退行性变,类脂质空泡减少。知柏地黄丸治疗后,肾上腺各层增厚,退行性变细胞明显减少,类脂质空泡较多。结论:知柏地黄丸能显著提高激素性肾阴虚幼龄大鼠血浆CORT、ACTH、CRH及肾上腺指数,故证实该药可有效拮抗外源性糖皮质激素对HPA轴的抑制作用。

Objective： To study the effects of ZhiBaiDiHuangWan on levels of CORT,ACTH,CRH in blood and weight-index,tissue structure of adrenal in young rats model of Yin defiency. Methods：A deficiency of Yin young rat model was established using high dose hydrocortisone. 75 young rats wee divided into 5 groups randomly： normal group, model group, high, low dose of ZhiBaiDiHuangWan group and LiuWeiDiHuangWan group,the effect of high,low dose of ZhiBaiDiHuangWan and LiuWeiDiHuangWan were studied. Results： in model group,the contents of cGMP in blood was much lower,the ratio of cAMP/cGMP was much higher than normal group. ZhiBaiDiHuagWan and LiuWeiDiHuangWan could significantly increase CORT ,ACTH and CRH in blood, also increase weight-index of adrenal, while the increasnlent of high dose of ZhiBaiDiHuangWan was higher than LiuWeiDiHuangWan. Conclusion： ZhiBaiDiHuangWan can obviously improve CORT, ACTH,CRH in blood and weight-index of adrenal, so it was suggested that ZhiBaiDiHuangWan can significantly oppose the retraint from Gc to HPA.