模拟水体镉污染对水稻幼苗某些酶类活性的影响

Effect of simulative water cadmium pollution on some enzymes activity in rice seedlings

试验采用不同浓度（0.0、0.1、1.0、10、100 mg/L）的Cd^2＋溶液模拟水体镉污染,研究Cd^2＋对水稻种子萌发和幼苗生长期间两类酶活性的影响.结果表明,参与物质代谢的3种酶活性受Cd^2＋抑制,且随Cd^2＋处理浓度的升高而明显降低,降低的幅度是：淀粉酶（AMY）10.44%～57.14%,脱氢酶（DHG）11.93%～53.39%,硝酸还原酶（NR）14.65%～54.06%.与此相反,Cd^2＋对抗氧化酶类活性却有明显的刺激作用：超氧化物歧化酶（SOD）活性提高32.84%～133.65%,过氧化物酶（POD）活性提高31.28%～133.66%,过氧化氢酶（CAT）活性提高33.22%～120.62%.抗氧化酶类消除了活性氧的氧化作用,从而避免或减轻了活性氧对细胞结构的破坏.

The effect of simulative water Cd^2＋ pollution on the activity of some enzymes during seed germination and seedling growth of rice were studied. The seeds and seedlings of rice were treated by 0, 0.1, 1, 10, 100 mg/L Cd^2＋ solution. The activity of three enzymes for substance metabolism were significantly inhibited by Cd^2＋. The activity of amylase （AMY）, dehydrogenase （DHG） and nitrate reductase （NR） decreased by 10.44% -- 57.14%, 11.93% -- 53.39% and 14.65% 54.06%, respectively. The Cd^2＋ stimulated antioxidant enzymes： activity of superoxide dismutase （SOD）, peroxidase （POD） and catalase （CAT） increased by 32.84% - 133.65%, 31.28% 133.66 % and 33.22 % - 120.62 %, respectively. tion of active oxygen and protected cell structure.