峨眉含笑基因组DNA提取及RAPD反应体系的优化

Genomic DNA extraction and optimization of RAPD analytic conditions of Michelia wilsonii

以峨眉含笑嫩叶为材料,研究了峨眉含笑基因组DNA提取方法及RAPD反应条件。结果表明:采用改良的CTAB方法,提取的DNA质量较高,适宜于RAPD-PCR分析。在25μL反应体积中,RAPD分析的优化反应体系为:1.5 mmol/L Mg2+、0.8 mmol/L dNTP、240 nmol/L随机引物、80 ng DNA、1.0 U Taq DNA聚合酶。

The method of genomic DNA extraction and the optimization of RAPD analytic conditions were studied in Michelia wilsonii. The results showed that high grade genomic DNA was obtained by the revised CTAB method. The optimal PCR system for RAPD analysis was as follows： 1.5 mmol/L Mg^2＋ , 0.8 mmol/L dNTP, 240 nmol/L random primer, 80 ng DNA template, 1.0 U Taq polymerase, in 25μL reaction system.