水中叶青双及其降解物的反相液相色谱测定研究

RP-HPLC determination of germicide MBAMT and its decomposition product in aquatic environment

用反相液相色谱法测定水中叶青双及其降解物2-氨基-5-巯基-1,3,4-噻二唑,平均回收率分别为91.7%和94.2%;对水样的最低检测浓度分别为7.19 nmol/L和2.87 nmol/L;叶青双与2-氨基-5-巯基-1,3,4-噻二唑的分离度为2.5。此方法在柱效和灵敏度上比硅胶柱液相色谱法有明显的提高。

This paper presented a method of reversed phase high performance liquid chromatography to determine the germicide N, N - methylene - bi - （2 - amino - 5 - mercapto - 1,3,4 - thidiazole） （MBAMT） and it＇s decomposition product 2 - amino - 5 - mercapto- 1,3,4 - thidiazole） （AMT）. Recoveries of MBAMT and AMT were 90% and 94% respectively. The detected limits of concentration for water sample were 7.19 nmol/1 for MBAMT and 2.87 nmol/1 for AMT. The resolution between the peaks of MBAMT and AMT was 2.5. The column efficiency and detected sensitivity of this method were significantly better in comparision with normal phase chromatography.