COX-2与VEGF-C在直肠癌中的表达及其在淋巴转移中的意义

The correlation between VEGF-C and COX-2 expression in human rectal cancers and its role in lymph node metastasis

目的探讨直肠癌中VEGF-C及COX-2表达的关系及意义。方法用COX-2特异性抑制剂NS-398及其催化产物PGE2作用于大肠癌LOVO细胞,应用Western blot方法检测VEGF-C蛋白表达。应用免疫组化方法检测45例直肠腺癌组织VEGF-C及COX-2表达情况。结果 NS-398 明显抑制LOVO细胞VEGF-C蛋白的表达。经PGE2作用后,VEGF-C蛋白表达上调。直肠癌组织中 VEGF-C及COX-2的表达具有明显相关性。结论COX-2上调VEGF-C表达,促进肿瘤淋巴转移。

Objective To investigate the correlation between VEGF-C and COX-2 expression in human rectal cancers and its significance in cancer metastasis. Methods VEGF-C expression was detected with Western blot in LOVO cells treated with NS-398 or PGE2. VEGF-C and COX-2 expression in 45 rectal adenocarcinomas was tested with immunohistochemistry. Results NS-398 inhibited the VEGF-C expression, and PGE2 up-regulated the expression of VEGF-C in a dosage-dependent way in LOVO cells. VEGF-C expression was significantly higher in adenocarcinomas with lymph node metastasis, and was related with the expression of COX-2 in 45 rectal adenocarcinomas. Conclusion COX-2 up-regulates VEGF-C and VEGF-C plays an important role in lymphatic metastasis of rectal cancers.