CO_2气腹对结肠癌细胞表面粘附分子表达影响的研究

The influence of CO_2 insufflation on the expression of adhesion molecules on colon cancer cell

目的探讨不同压强持续性CO2气腹对结肠癌细胞表面粘附分子表达的影响。方法建立体外气腹模型,选用人结肠癌细胞株SW1116,分别在不同压强医用CO2气体下暴露1h,使用流式细胞技术和实时荧光定量PCR检测粘附分子E-cadherin,ICAM-1,CD44,CD44v6,E-selectin的表达。结果荧光定量PCR结果显示SW1116经6mmHg压强的持续CO2气体处理后,ICAM-1和CD44v6出现表达增高;9mmHg及12mmHgCO2气体处理后,E-cadherin,CD44和CD44v6表达增高;15mmHgCO2气体处理后CD44v6表达增高,与处理前表达水平的差异均有统计学意义(P<0·05),上述粘附分子的表达在处理后72h内均会降至处理前水平(P>0·05),或低于处理前水平(P<0·05)。E-cadherin、CD44v6和ICAM-1随着压强增高,其表达量逐渐降低。结论不同压强CO2气腹能对肿瘤细胞表面的粘附分子表达产生一过性双向影响,随CO2气腹压强的增高,可抑制粘附分子的表达。

Objective To investigate the influence of CO2-insufflation on the expression of some adhesion molecules. Methods With an artificial pneumoperitoneum model in vitro, SWI 116 colon carcinoma cells were exposed to CO2-insufflation at different pressure （6, 9, 12 and 15 mmHg） for 1 hour. Control groups were exposed to room air. E-cadherin, ICAM-1, E-selectin, CD44 and CD44v6 were measured 0, 12, 24, 48 and 72 hours after CO2-insufflation using flowcytometry and the mRNA level of these adhesion molecules were detected by real-time PCR. Results In transcription level the expression of ICAM-1 and CD44v6 on SW1116 cell line increased significantly in 6mmHg CO2-insufflation group, in 9mmHg and 12mmHg group, E-cadherin, CD44 and CD44v6 increased significantly, and when the pressure was 15mmHg, CD44v6 increased （ P 〈 0. 05 ）, the expression of these adhesion molecules returned to or even less than the control level by 72 h（ P 〈0.05 ）. With the CO2-insufflation pressure ascending, the increasing expression of the E-cadherin, CD44v6 and ICAM-1 were inhibited in some time points （P 〈0. 05）. In translation level we got the similar result. Conclusion The expression of these adhesion molecules was affected temporarily and bi-directionally after the continuous CO2-insufflation. The higher pressure of CO2-insufflation plays an important role in inhibiting the expression of the adhesion molecules.