大鼠颈淋巴引流阻断后急性期内脑结构、微区脑血流量和体感诱发电位的变化

Alteration of Morphology, Micro-region Cerebral Blood Flow and Somatosensory Evoked Potential Following Cervical Lymphatic Blockage in Rats

通过结扎并摘除颈深淋巴结造成脑淋巴引流障碍,观察不同时间脑结构的变化,同时检测大脑皮层微区血流量和体感诱发电位的动态变化。结果示:光镜下见组织间隙增宽液体瘀滞神经元变性、坏死,有大量吞嗜细胞浸润,形成"卫星现象";电镜下见神经元肿胀坏死,大胶质细胞肿胀,线粒体等亚细胞结构变化明显。还发现颈淋巴引流阻塞后1天、5天和7天大脑皮层微区血流最显著降低和阻塞后5天、7天体感诱发电位潜伏期明显延长。结果表明阻断颈淋巴引流对脑结构有明显影响,其结构变化构成了"淋巴性脑水肿",开拓了脑水肿研究的新领域;同时表明颈淋巴引流阻断与脑组织供血有关;体感诱发电位变化是诊断淋巴性脑水肿的一个重要指标。

Cerebral Lymphatostasis was performed by occluding and removing the. deep cervical nodes in rats, morphological alteration was observed at different times; the changes of somatosensory evoked potential(SEP) and micro-region cerebral blood flow (MrcBF) were measured at different days simultaneously. The results showed that cerebral tissue space prolongation, increased significantly interstitial fluid, neuronal degeneration and necrosis, diffused phagocytes and satellitosis were observed under microscope; neuronal swell and necrosis, glial cell swell, apparent subcellar changes such as mitochondrion were seen under electronscope; MrcBF decreased significantly at 1, 5, 7, days (P<0.05) and the absolute latency of SEP delayed significantly at 5, 7 days after cervical lymphatic blockage. All of results suggest that cerebral lymphatostasis could lead to cerebral structre changes which were named as lymphatostatic encephaloedema as well as explored a new research field of cerebral edema; cerebral lymphatostasis has a relation to cerebral blood supply and the change of SEP was an important parameter in diagnosing lymphatostasis encephaloedema.