PCR法检测乳腺癌患者血清抗p53蛋白抗体的研究

Detection of seral p53 antibody in patients with breast carcinoma by immuno-polymerase chain reation

目的为乳腺癌诊断提供血清学新方法。方法用免疫-聚合酶链反应(i mmuno-polymerasechain reation,PCR)法检测血清抗p53蛋白(一种抑癌基因)抗体,酶免疫组化方法检测组织p53蛋白表达。结果乳腺癌患者血清抗p53蛋白抗体阳性率为39·5%,而非癌患者和正常人血清抗p53蛋白抗体均为阴性,乳腺癌患者血清中抗p53蛋白抗体显著高于非癌患者和正常人(P<0·01)。p53蛋白阳性表达的乳腺癌患者抗p53蛋白抗体阳性率为64·2%,明显高于p53蛋白阴性表达组,血清p53抗体测定与p53蛋白表达密切相关(P<0·01)。结论检测血清抗p53蛋白抗体是检测组织p53蛋白理想的替代工具,抗p53蛋白抗体可以作为乳腺癌血清学诊断新标志,用于乳腺癌的普查和早期诊断。

Objective To present a new seriological method for diagnosing breast carcinoma. Methods Seral anti-p53 antibody was detected with immuno-polymerase chain reation（ Immuno-PCR）. The expression of p53 in tissue was detected with enzymeimmu-nohistochemistry technique. Results The positive rate of anti-p53 antibody in serum of breast carcinoma was 39.5 % and anti-p53 antibody was not found in non-carcinoma and normal controls. The positive rate of anti-p53 antibody was higher than that of non-carcinoma and normal controls（ P 〈 0.01 ）. The positive rate of anti-p53 antibody in positive p53 expression was 64.2 % and was higher than that in negative p53 expression. Seral anti-p53 antibody was related to p53 expression in tissue obviously（ P 〈 0.01 ）. Conclusion Detecting seral anti-p53 antibody is an alternative tool for detecting p53 expression in tissue. Seral anti-p53 antibody is a new marker for diagnosing breast carcinoma and can be used in early dianosing breast carcinoma.