中药抗炎作用与对大鼠腹腔巨噬细胞产生白三烯B_4及5－HETE的影响

EFFECT OF ANTI-INFLAMMATORY ACTION OF CHINESE HERBS ON THE SYNTHESIS OF LTB4 AND 5-HETE OF PERITONEAL MACROPHAGES IN RATS

本文建立了提取与测定大鼠腹腔巨噬细胞（ＰＭφ）生物合成白三烯Ｂ_４（ＬＴＢ_４）及５－羟基二十碳四烯酸（５－ＨＥＴＥ）的高效液相色谱法，并研究了４个单味中药抗炎作用与对ＰＭφ产生ＬＴＢ_４及５－ＨＥＴＥ的影响。结果表明大黄、厚朴、白头翁及败酱草提取物对大鼠ＰＭφ合成以上二种介质均有明显抑制作用。对炎性介质ＬＴＢ_４产生的抑制率（％）分别为：大黄素（终浓度取５．０×１０^（－６）ｍｏｌ／Ｌ）为８８．０％；厚朴为９９．８％；白头翁为９４．９％及败酱草为５７．９％（以上终浓度均取５．９×１０^（－２）ｇ／Ｌ）。

LTB4 and 5-HETE were determined by High Performance Liquid Chromatography,The anti-inflammatory action of four extractions from Chinese herbs including Emodin,Magnolia Officinalis,Pulsatilla Chinensis and patrinia vilosa,was investigated for its effect on the synthesis of LTB4 and 5-HETE released form peritoneal macrophages stimulated by calcium ionophore A23187,The results showed that the four herb extractions significantly inhibited the biosynthesis of both LTB4 and 5-HETE,the inhibite rate was from 60%to 98%as same as that of flufenamic acid.We suggested that the mechanism of antiinflammatory action of these agents may be related to the suppresion of lipoxygenase.