文蛤和波纹巴非蛤最佳抗冻剂配方的实验研究

Experimential Study on Optimum Processing Technique of Preventing the Freezing Denatureation of Meretrix Linnaeus and Paphia Undulata

以盐溶性蛋白溶解度、Ca2+ATPase活性和pH值为指标,通过正交实验L9(34)研究,探讨了防止文蛤和波纹巴非蛤蛋白质冷冻变性的最佳抗冻剂配方。实验结果表明,文蛤的最佳抗冻剂配方为焦磷酸钠0.1%、三聚磷酸钠0.1%、蔗糖2%、山梨醇3%;波纹巴非蛤的最佳抗冻剂配方为焦磷酸钠0.1%、三聚磷酸钠0.15%、蔗糖4%、山梨醇3%。在添加最佳抗冻剂后文蛤的Ca2+ATPase活性、盐溶性蛋白溶解度和pH值分别提高了26.4%、25.5%和2%,波纹巴非蛤则分别提高了21%、38.5%和2.5%。

To study the optimum of preventing the frezz denaturalization of Meretrix Linnaeus and Paphia Undulata, orthogonal design L9（34） is used. Salt-solubility,Ca^2＋-ATPase activity and pH are used as indexes. The results showed with respect to Meretrix Linnaeus, the optimum was Sodium pyrophosphate （0.1% ）, Sodium tripolyphosphate（0.1% ） ,sucrose（2% ） ,sorbite（3%） ; As for Paphia Undulata, the optimal condition was Sodium pyrophosphate （0.1% ）, Sodium tripolyphosph ate （ 0.15 % ）, sucrose （ 3 % ）, sorbite （ 3 % ）. When Cryoprotection was added, salt-solubility,Ca^2＋-ATPase activity and pH of Meretrix Linnaeus were improved by 26.4 % ,25.5 % and 2 % , while in Paphia Undulata they were improved by 21% ,38.5 % and 2.5 %.