实时定量PCR在细菌移位研究中的潜力

Potential Application of Real-time Quantitative PCR in Bacterial Translocation Study

实时定量PCR(RQPCR)是一种新型核酸定量技术,具有实时监测、快速、灵敏、精确、特异等特点。与原有PCR技术相比,其最主要的优势是能对待测样本起始PCR反应模板进行准确定量,扩大了PCR的应用范围。目前在细菌移位研究中,对肠黏膜屏障损伤的早期诊断一直没有很理想的方法。RQPCR能准确定量外周血中细菌DNA,有利于对肠黏膜屏障损伤的早期诊断和损伤程度评估。

Real-time quantitative polymerase chain reaction （RQ-PCR） is a rapid, sensitive, specific, and precise technique for nucleic acid quantification. In contrast to the conventional PCR techniques, it can accurately quantify the initial templates in an amplification reaction and thus extend the application scope of PCR. RQ-PCR can also accurately quantify bacterial DNA in peripheral blood samples, which is helpful for the early diagnosis and injury evaluation of gut barrier damage.