β－内啡肽和强啡肽A对急性缺血大脑皮层Ca^（2＋）、Mg^（2＋）含量及皮层血流量的影响

The Influence of β-EP and DynA to Content of Calcium , Magnesium and rCBF during Acute Ischemic Cortical Edema in Mongolian Gerbils

采用原子吸收分光光度法测定侧脑室微量注射β－内啡肽（β－ＥＰ）、强啡肽Ａ（ＤｙｎＡ）和纳洛酮（Ｎｘ）后，对于沙土鼠急性缺血大脑皮层Ｃａ^（２＋）、Ｍｇ^（２＋）和Ｈ＿２Ｏ含量的影响，利用氢清除法观察了动物大脑皮层局部血流量以及静脉注射β－ＥＰ和Ｎｘ后，对于皮层局部血流量的影响，并探讨了β－ＥＰ和ＤｙｎＡ在沙土鼠急性缺血性皮层水肿中的作用及其机理。结果表明：（１）侧脑室注射β－ＥＰ后，在缺血皮层水肿加重的同时，大脑皮层Ｃａ^（２＋）含量升高非常显著（Ｐ＜０．０５），Ｍｇ^（２＋）含量降低（Ｐ＜０．０５），而侧脑室注射ＤｙｎＡ后大脑皮层Ｃａ^（２＋）含量明显降低（Ｐ＜０．０５），Ｍｇ^（２＋）含量升高。（２）侧脑室注射Ｎｘ后沙土鼠缺血大脑皮层Ｃａ^（２＋）含量明显降低（Ｐ＜０．０１）的同时，皮层组织Ｍｇ^（２＋）含量显著增加（Ｐ＜０．０５），缺血性皮层水肿有非常明显的减轻（Ｐ＜０．０１）。（３）尾静脉注射β－ＥＰ、ＤｙｎＡ和Ｎｘ后，动物大脑皮层局部血流量和相应对照组相比无显著改变。提示β－ＥＰ加重或促进缺血性脑水肿的作用与大脑皮层局部血流量的改变无明显相关，内源性阿片肽在缺血性脑水肿中的作用及其差异可能和它们调节组织内?

It was studied that the effect of β-endorphin(β-EP),dynorphin A1-13(DynA)and naloxone (Nx)injected into ventriculus lateralis on calcium(Ca2+), magnesium (Mg2+)and water(H2O)content of acute ischemic cortex and examined that regional cerebral blood flow (rCBF ) changed when β-EP, DynA and Nx were intravenously injected. The purpose of this study was to research the mechanism of the role β-EP and DynA in acute cerebral ischemia of gerbils. The calciurn and magnesium contents were measured by atomic absorbtion spectrometry(AAS), and the rCBF was analysed using hydrogen gas clearance technique. It was observed that:1 )Calcium content of ischemic cortex increased remarkably(P<0.05 ), magnesium content decreased ,as well as cortical edema worsened(P<0. 01 )in β-EP group , while in DynA group calcium content and H20content of ischemic cortex decreased significantly. 2) When Nx was injected into ventriculus lateralis , calcium and H2O content of ischemic cortex decreased and magenesium content increased remarkably. 3) The rCBF was unchanged,while β-EP, DynA and Nx were intravenously injected as compareed with that of control groups.The results suggested that the different role of β-EP and DynA in acute cerebral ischemia of gerbils might related with calcium and magenesium metabolism which were regulated by endogenous opiate peptides.