摘要
A novel three-liquid-phase extraction system （TES） composed of butyl acetate, block copolymer polyethylene oxide-polypropylene oxide-polyethylene oxide and ammonium sulphate aqueous solution [（NH4）2SO4] as top, middle, and bottom phase, respectively, has been developed. The copolymer recycling and partitioning behavior of penicillin V has been studied in this system. Results show that the copolymer could be purified and recycled and penicillin V of the filtrated ferment broth could be partitioned unevenly among the phases and purified in the top phase of this TES. About 90 wt.% of penicillin V could be distributed into the top phase around pH 2.5 and only less than 0.1 wt.% left in the bottom phase.
A novel three-liquid-phase extraction system （TES） composed of butyl acetate, block copolymer polyethylene oxide-polypropylene oxide-polyethylene oxide and ammonium sulphate aqueous solution [（NH4）2SO4] as top, middle, and bottom phase, respectively, has been developed. The copolymer recycling and partitioning behavior of penicillin V has been studied in this system. Results show that the copolymer could be purified and recycled and penicillin V of the filtrated ferment broth could be partitioned unevenly among the phases and purified in the top phase of this TES. About 90 wt.% of penicillin V could be distributed into the top phase around pH 2.5 and only less than 0.1 wt.% left in the bottom phase.
基金
Innovative Research Group Science Fund (No. 0221603), the National Natural Science Foundation (No. 20406021) and he National Natural Science Key Foundation (No. 20236050) of China
