PCR技术检测复发性呼吸道乳头状瘤组织中的HPVDNA

The Correlation Between Recurrent Respiratory Papllomatosis and HPV Infection

应用多重引物ＰＣＲ技术对３６例复发性呼吸道乳头状瘤病（ＲＲＰ）和９例声带息肉石蜡包埋组织中的ＨＰＶ６／１１、１６、１８ＤＮＡ进行了检测。结果发现：①ＲＲＰ组织中ＨＰＶ６／１１ＤＮＡ的阳性率为６９．４％（２５／３６），其中喉乳头状瘤、口咽部乳头状瘤和鼻前庭乳头状瘤组织中ＨＰＶ６／１１ＤＮＡ的阳性率分别为７０．４％（１９／２７）、６６．７％（４／６）和６６．７％（２／３）；所有ＲＲＰ标本中均未发现特异性的ＨＰＶ１６和ＨＰＶ１８ＤＮＡ；②９例声带息肉标本中四种型别的ＨＰＶＤＮＡ全部阴性。结果表明：ＲＲＰ的发病与ＨＰＶ６／１１感染密切相关，多重引物ＰＣＲ技术检测ＨＰＶＤＮＡ具有敏感性高、特异性强和操作快速、简便等优点。

Formalin-fixed,paraffin-embeded tissue from 36 patients with recurrent respiratory papillomatsis(27 laryngeal pa-pillomas,6 oropharyngeal papillornas and 3 nasal vestibular papillomas ) and 9 patients with cordal polyps was evaluated for thepresence of HPV6/11、16、18 DNA using polymerase chin reaction(PCR) with multlple primers. HPV6/11 DNA was found in 19of 27 laryngeal papillorns,4 of 6 oropharyngeal papillornas and 2 of 3 nasal vestibular papillomas. HPV16、18 DNA was negativein all papilloma specimens.No HPV DNA was found in cordal polyps,These resuIts showed that HPV was liighly implicated inthe pathogenesis of recurrent respiratory papillomatosis.The multiple primer PCR techinique is a useful tool for thdetection of HPV.