摘要
本文对人胃低分化液液腺癌ＭＧｃ８０－３及其克隆株Ｃ２、Ｃ３、Ｃ５、Ｃ１１体外侵袭能力进行定量研究。以鸡胚心肌作为肿瘤细胞侵袭的靶器官，用半自动数字图像分析仪检测肿瘤细胞对其侵袭能力。结果表明：ＭＧｃ８０－３母系及其克隆株的侵袭力不同，第１天和第３天时Ｃ２、Ｃ１１的侵袭力明显高于ＭＧｃ８０－３及Ｃ３、Ｃ５，到第５和第７天时，ＭＧｃ８０－３及其克隆重株的侵袭力无明显不同。各细胞系（株）２４小时开始粘附、包绕或侵袭心肌组织，随着培养时间的延长，肿瘤细胞所占据的面积呈进行性增大。本实验验证了癌细胞在侵袭过程中有粘附、溶解破坏、运动功能及分裂增生能力。人胃低分化粘液腺癌ＭＧｃ８０－３及其克隆株Ｃ２、Ｃ３、Ｃ５、Ｃ１１的体外侵袭力明显不同，其体外侵袭力，特别是在早期ＭＧｃ８０－３和克隆株之间的侵袭能力差异较大。
Quantitative study were performed on invasion in organ culture of parental MGc80-3 and its clone strains,C2,C3C,5 and C11.In order to examine the different invasion ability of different cell strains in organ culture,MGc803 and C2,C3,C5,C11were confronted with 1 mm round precultured embryonic chick heart fragments,After co-culture at 37℃,in a humidified atmo-sphere with 5%CO2,the quantitative change of tumor invasion were measured by the computer-image processing system.The re-sults showed that the invasion of MGC80-3 and its clone strains cells was progressive,In the progression of invasion,all cell lines(strains)had the ability of adhesion,destruction and motion,In addition,C2 and C11 had a higher invasive ability than MGc80-3,C3,C5 in the first and the third day.
