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串珠素对大鼠平滑肌细胞增殖的影响及其机制的研究 被引量：1

Effect of Perlecan on Vascular Smooth Muscle Cells Proliferation and Mechanism
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摘要 目的观察串珠素对于大鼠血管平滑肌细胞(vascularsmoothmusclecells,VSMCs)增殖反应的影响以及细胞信号转导机制。方法以3H-TdR掺入法测定VSMCs增殖,Westernblot方法检测FAK表达。结果Perlecan加强碱性成纤维细胞生长因子(basicfibroblastgrowthfactor,bFGF)引起的VSMCs增殖反应,并诱导粘着斑激酶(focaladhesionkinase,FAK)表达上调。Perlecan抗体消除Perle-can促进bFGF致VSMCs增殖反应,并抑制FAK表达。结论串珠素增强bFGF致大鼠平滑肌细胞的增殖反应,其机制涉及FAK表达上调。 Objective To investigate the effect of pedeean on enhanced vascular smooth muscle cells proliferation and the signaling transduetion mechanisms. Methods VSMCs culture in vitro and 3H-TdR incorporation was estimated as proliferation of VSMCs; The expression of FAK was detected by Western blot. Resulls Perlecan enhanced the proliferation of VSMCs stimulated by bFGF and up- regulated FAK expression（P 〈0.05）. After preconditioned antibody of perlecan, the function that perlecan enhanced the proliferation of VSMCs stimulated by bFGF was abolished, and FAK was inhibited （P 〈0.05）. Conclusion Perlecan can enhance the proliferation of VSMCs stimulated by bFGF. This function was related to up- regulation of FAK.
作者 付艳 刘秀华 蔡莉蓉
机构地区 [ 解放军总医院病理生理研究室
出处 《中国微循环》 北大核心 2006年第2期108-110,共3页 Journal of Chinese Microcirculation
基金 国家自然科学基金(30370569) 解放军总医院科研基金(01YM30)资助
关键词 串珠素 血管平滑肌细胞 增殖 粘着斑激酶 Perlecan VSMCs Proliferation FAK
分类号 R322.1 [医药卫生—人体解剖和组织胚胎学]
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参考文献6

  • 1刘秀华.串珠素的生理与病理生理作用[J].生理科学进展,2004,35(1):83-85. 被引量：7
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  • 3Belknap JK,Weiser MC,Grieshaber SS,et al.Relationship between perlecan and tropoelastin gene expression and cell replication in the developing rat pulmonary vasculature[J].Am J Respir Cell Mol Biol.1999,20(1):24～34.
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二级参考文献11

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共引文献12

同被引文献8

  • 1Aviezer D,Hecht D,Safran M,et al.Perlecan,basal lamina proteoglycan,promotes basic fibroblast growth factor-receptor binding,mitogenesis and angiogenesis[J].Cell.1994,79(6):1005～1013
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  • 6Furuta GT,Dzus AL,Taylor CT,et al.Parallel induction of epithelial surface-associated chemokine and proteoglycan by cellular hypoxia:implications for neutrophil activation[J].J Leukoc Biol.2000,68(2):251～259
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中国微循环
2006年 第2期

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