骨多肽生长素、珊瑚骨、地榆诱导鼠额面骨再生的研究

Experimental study on bone regeneration induced by bone active polypeptide,coralline,Sanguisorba officinalis

目的评价骨多肽生长素(BAP)应用于小白鼠额面骨缺损区诱导骨再生修复的效果。方法昆明小白鼠63只随机分为3组,实验组植入骨多肽生长素复合珊瑚骨,对照A组植入珊瑚骨,对照B组植入珊瑚骨复合地榆。观察骨组织形态变化和新生骨结构,计量每视野新生成骨细胞健康数;在透射电镜下观察再生骨的超微结构变化。结果术后7 d,骨缺损区周围有破骨细胞、间充质细胞和大量炎性细胞浸润,骨变性坏死。术后28 d,实验组可见成纤维细胞、毛细血管生长活跃,可见骨样组织和大量骨组织;对照A组可见骨缺损区周围有间充质细胞和成骨细胞;对照B组可见成纤维细胞、毛细血管生长活跃,骨缺损区周围有成骨细胞。术后90 d,实验组可见新生骨组织范围扩大,钙化程度加强,几乎接近正常骨质结构;对照A组、B组皆可见骨缺损边缘有较多新骨沉积。计量3组新生成骨细胞数,运用单因素方差分析及两两比较,实验组与各对照组差异有显著性。结论应用骨多肽生长素复合载体材料珊瑚骨可促进骨缺损的修复。

Objective To evaluate the effects on the frontal bone regeneration of the mice induced by bone active polypeptide （BAP）, and to study the mechanism of bone regeneration induced by BAP. Methods 63 Kunming mice were randomly divided into 3 groups. One was experimental group, and the two were control groups. The mice were operated. Bone defects of 3 mm × 2 mm × 1 mm in site were created on frontal bone by using local anesthesia, and then the defects were filled with BAP composite coralline in the experimental group, only coralline in the control group A and eoralline composite Sanguisorba officinalis （SO） in the control group B. Postoperatively, the mice were killed at intervals of 7, 28 and 90 days, and the specimens were examined histopathologically by light microscope and transmission electron microscope. Osteoblasts were counted and studied quantitatively by analysis of variance（ANOVA） for comparison in each group. Results 7 days of postoperation, it was revealed that the bone defects were infiltrated by the white blood cells, degeneration and necrosis in all groups; after 28 days, the inflammation was absorbed, fibroblasts and new fine capillaries were developing actively, and new bone formation was also found in the experimental group. The inflammation was absorbed in the control group A, and in the control group B, fibroblasts and many new capillaries were found. After 90 days, ossification was obvious in the experimental group. Osteoblasts were counted, P 〈 0.01. Statistical analyses showed difference between the experimental and control groups. Conclusion The frontal bone regeneration in the mice can be induced by BAP, and BAP composite coralline was more remarkable. The mechanism of bone regeneration induced by BAP is that BAP is a variety of growth factors.