质粒缺口-连接酶链反应-ELISA法检测重庆地区围生期孕妇沙眼衣原体感染及基因分型

Study on the perinatal Chlamydia trachomatis infection in Chongqing region by plasmid Gap-LCR-ELISA and genotype assay

目的:用质粒缺口-连接酶链反应(Gap-LCR)-ELISA法研究重庆地区围生期孕妇沙眼衣原体(CT)感染的现状及流行的主要基因型别,为其疫苗的研制提供分子流行病学依据。方法:以512例孕妇的首段尿(FVU)及其配对新生儿的鼻咽拭子为标本,用质粒Gap-LCR-ELISA法检测CT感染者,对阳性母、婴配对标本DNA行omp1-VS4-PCR扩增,产物行双链DNA直接测序并作基因分型。结果:所检重庆地区孕妇CT感染率为7.42%,母婴垂直传播率为15.79%,以阴道分娩为主要传播途径。6对阳性母婴配对标本的CT基因序列一致,其中5对为E型,1对为H型,再次证实了CT的垂直传播途径。结论:质粒Gap-LCR-ELISA法可用于围生期无症状CT感染孕妇的大规模流行病学调查。重庆地区围生期CT流行型别仍以E型为主。

Objective： To study the current status of perinatal Chlamydia trachomatis （CT） infection in Chongqing region by plasmid Gap-LCR-ELISA and genotype assay, and then to provide clues for CT vaccine research. Methods： Five hundred and twelve urine specimens from pregnant women and their newborns＇ nasopharyngeal swabs were taken as the samples, which were examined by plasmid Gap-LCR-ELISA to detect CT infection, ompl-VS4-PCR and DNA sequence analysis were con- ducted for the CT positive maternal-infant pairs to genotyping the CT strains. Results： The prevalence of CT infection among pregnant women in Chongqing was 7.42% and the vertical transmission rate was 15.79 %, among which the vaginal delivery was the main route. The genotyping showed that 5 maternal-infant pairs were type E, with the remainder type H, which once again confirmed the vertical transmission route of CT infection. Conclusions： Plasmid Gap-LCR-ELISA is a good method for large-scale epidemic screening for perinatal asymptomatic CT infections. The prevalent CT strain in Chongqing is still type E.