尖锐湿疣、鲍恩样丘疹病、Bowen病及外阴鳞状细胞癌皮损中端粒酶的表达

Expression of telomerase in skin lesions of condyloma acuminatum,bowenoid papulosis,Bowen's disease and vulvar squamous carcinoma

目的探讨端粒酶在正常组织、良性增殖性疾病及恶性肿瘤中的表达情况及其临床意义。方法采用免疫组化方法检测皮损中端粒酶逆转录酶(hTERT)蛋白的表达情况,并对所有鲍恩样丘疹病和Bowen病病例进行组织病理分析。结果正常人对照组、尖锐湿疣组及外阴鳞状细胞癌组中任两组间hTERT表达强度差异均有统计学意义,其阳性率依次增高。鲍恩样丘疹病组较尖锐湿疣组hTERT表达强度高,两组差异有统计学意义；虽与Bowen病组比较差异无统计学意义,但较外阴鳞状细胞癌组表达强度低,差异有统计学意义。组织病理上,Bowen病比鲍恩样丘疹病细胞异形性更明显。结论端粒酶在正常组织、良性增殖性疾病及恶性肿瘤中的表达强度呈梯度升高,提示端粒酶激活在细胞增生及永生化中均发挥重要作用。

Objective To study the expression of telomerase and its possible clinical significance in patients with condylorna acuminatum, bowenoid papulosis, Bowen＇s disease and vulvar squamous cell carcinoma. Methods The human telomerase reverse transcriptase （ hTERT ） was detected by immtmohistochemistry technique in all patients. Histopathological analysis was done in patients with bowenoid papulosis or Bowen＇s disease. Results There was a significant difference in the expression of hTERT among normal tissue, condyloma acuminatum and squamous carcinoma, and the expression rate increased successively from normal control to squamous carcinoma. In bowenoid papulosis, the expression of hTERT was significantly higher as compared to that in condyloma acuminatum, significantly lower as compared to that in squamous carcinoma, and was as the same as that in Bowen＇s disease. Histopathologically, the degree of cell atypia was significantly higher in Bowen＇s disease than that in bowenoid papulosis. Conclusions The expression of telomerase increases successively from normal skin, benign tumors to malignant carcinomas, which suggests that the expression of telomerase may play an important role in both cell proliferation and immortalization.