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小麦低分子量谷蛋白亚基基因5′侧翼保守序列染色体组特异性DNA变异的分析及验证 被引量：1

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摘要 根据33个低分子量麦谷蛋白亚基(LMW-GS)基因5′侧翼序列的相似性进行聚类分析,可将其划分成8个类群,这与基于N末端推导氨基酸序列进行的类群划分结果完全一致.序列比对发现,各类群基因5′侧翼保守序列间存在DNA多态性,共发现34个多态性位点,其中18个为潜在单核苷酸多态性位点(SNPs,singlenucleotidepolymorphisms).除1个LMW-GS类群之外,其余7个类群的5′侧翼序列均具有类群特异性DNA变异位点.根据类群间的DNA多态性对这7个类群设计了特异引物,利用普通小麦(TriticumaestivumL.)品种中国春及其第1同源群双端体系对其进行染色体定位分析,揭示了1AS,1BS和1DS上分别有第2,1和4类群.对PCR产物的克隆测序进一步验证了不同染色体组上的LMW-GS基因类群间5′侧翼序列具有特异性.这些结果表明,LMW-GS基因的编码区及其5′侧翼保守序列可能是协调进化的.本文报道的7对引物可对7类LMW-GS基因的完整编码区进行特异扩增,因而能在小麦复杂的遗传背景下有目的地对某一类LMW-GS基因进行分离克隆,这有助于弄清单个LMW-GS对小麦品质的贡献.同时,在小麦育种中,这些标记对于有效地选择与品质密切相关的LMW-GS组分有一定应用价值.
作者 龙海 魏育明 颜泽洪 Bernard Baum Eviatar Nevo 郑有良
机构地区 四川农业大学小麦研究所 Eastern Cereal and Oilseed Research Centre Institute of Evolution
出处 《中国科学（C辑）》 CSCD 北大核心 2006年第2期118-126,共9页 Science in China(Series C)
基金 国家高技术研究发展计划项目(批准号:2003AA207100) 国家自然科学基金项目(批准号:30300219 30571163) 高等学校优秀博士学位论文作者专项资金项目(批准号:200357 200458) 长江学者和创新团队发展计划项目(IRT0453)资助
关键词 小麦 LMW-GS基因 5′侧翼序列 DNA多态性 SNP 基因克隆
分类号 S512.1 [农业科学—作物学] S532 [农业科学—作物学]
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共引文献90

同被引文献27

  • 1王志清,龙海,郑有良,颜泽洪,魏育明,兰秀锦.西藏半野生小麦LMW-GS基因的克隆及序列分析[J].Acta Genetica Sinica,2005,32(1):86-93. 被引量：12
  • 2孙学永,马传喜,司红起,张琪琪,乔玉强.中国小麦微核心种质低分子量麦谷蛋白Glu-A3位点等位基因的PCR检测[J].分子植物育种,2006,4(4):477-482. 被引量：13
  • 3逄孝云,许瑞瑞,王宪泽.普通小麦编码LMW-1、LMW-2基因的克隆及其与品质关系的研究[J].生物技术通报,2007,23(4):97-101. 被引量：1
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中国科学（C辑）
2006年 第2期

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