纤维环穿刺诱导椎间盘退变动物模型的实验研究

Construction of rabbit model of intervertebral disc degeneration induced by needle puncture in anulus fibrosus

目的:探讨纤维环穿刺诱导椎间盘退变建立动物模型的可行性。方法:新西兰大白兔24只,用持针器夹持18G皮肤穿刺针从左前外侧刺入L3/4、L4/5、L5/6椎间盘的纤维环,深度控制在5mm。术前及术后3、6、10周对造模后的椎间盘及对照的椎间盘(L2/3)行MRI检查,并行免疫组化及组织学观察。结果:术后第3周到第10周,造模后的椎间盘MRIT2WI信号呈现持续减弱趋势,免疫组化及组织学观察发现髓核细胞的数量及Ⅱ型胶原含量较对照间盘进行性减少(P<0.01)。结论:纤维环穿刺法可以诱导兔椎间盘的缓慢退变,为研究椎间盘的退行性变提供有效的动物模型。

Objective：To establish a slowly progressive,reproducible rabbit model of intervertebral disc degeneration induced by puncturing the anulus fibrosus with needles of defined gauges.Method：The L3/4,L4/5 and L5/6 lumbar intervertebral discs of 24 New Zealand white rabbits were stabbed by 18-gauge hypodermic needle into a depth of 5mm in the left anterolateral anulus fibrosus.Magnetic resonance imaging scans,immunohistochemical and histologic analyses of the stabbed discs and intact L2/3 discs were performed preoperatively and at 3,6,10 weeks respectively after surgery.Result：In the magnetic resonance imaging,the stabbed discs exhibited a progressive decrease of signal intensity in T2-weighted images which start at 3 week after stabbing and last for 10 weeks.Immunohistochemical and histologic analyses revealed progressive decrease of chondrocyte-like cells and type Ⅱ collagen （P〈0.01）.Conclusion：The stabbing approach results in a slowly progressive intervertebral disc degeneration in rabbit model.This model is available for studying the status intervertebral disc degeneration.