锌指基因ZNF268两个新的差异剪接本克隆及鉴定

Cloning and Characterization of Two Novel Alternatively Spliced Transcripts of ZNF268

ZNF268是一个典型的KRAB类锌指基因，含有24个C2H2型锌指基序和一个KRAB功能域，它在人胚胎发育早期表达，并与胚胎肝脏的发育相关。通过免疫组化的方法，发现ZNF268在3—5周龄人胚的造血干细胞中有表达；进一步通过RT—PCR的方法检测发现ZNF268在健康成人全血细胞中也有表达，并且表达有4个差异剪接本，从中克隆得到两个新的ZNF268差异剪接本ZNF268c和ZNF268d。推测ZNF268和血细胞生长发育相关。

ZNF268 gene encodes a typical KRAB-containing zinc finger protein, which has an amino-terminal KRAB domain and 24 carboxyl-terminal （32 H2 zinc finger motifs, There is evidence that ZNF268 is expressed in human early embryos, and that it played a role in the development of human fetal liver. In this study, we used immunohistochamistry to show that ZNF268 was also expressed in hematopoietic stem cells in 3-5 week-old human embryos. ZNF268 expression was also detected in the blood cells from 7 healthy adult donors by RT-PCR. Furthermore, 4 alternative transcripts were detected in the 7 samples. By cloning and sequencing, two novel transcripts, ZNF268c and ZNF268d were identified. We predict that there is association between ZNF268 and the development of hematopoietic cells.