胃癌组织Maspin,uPA,MMP-7表达的意义

Significance of Maspin, uPA and MMP-7 expression in human gastric carcinoma

目的:观察胃癌及正常胃黏膜Maspin,uPA, MMP-7表达的意义．方法:应用免疫组化SP法检测胃管状腺癌30 例,胃印戒细胞癌30例,正常胃黏膜组织20例中Maspin,uPA,MMP-7的表达情况．结果:在胃管状腺癌中Maspin,uPA,MMP-7 阳性表达率分别为50％,70％和80％;胃印戒细胞癌中阳性表达率分别为46．7％,76．7％和 90％;正常胃黏膜组织中阳性表达率分别为 90％,35％和30％．Maspin的表达与浸润深度、淋巴结转移相关,而与肿块的大小和TNM分期无关．uPA和MMP-7的表达与浸润深度、淋巴结转移、TNM分期相关,而与肿块的大小无关．Maspin的表达与uPA和MMP-7的表达呈负相关(P=0．012,r=-0．322;P=0．008,r= -0．341);uPA的表达与MMP-7的表达呈正相关 (P=0．034,r=0．274)．结论:Maspin在胃癌中表达下调,uPA和 MMP-7在胃癌中过表达,他们在胃癌的浸润转移中起重要作用,可作为反应胃癌病理生物学行为的有效指标．

To invesfigate the expression of Maspin, urokinase-type plasrninogen activator （uPA） uPA and Matrix metal loproteinase-7 （MMP-7） and their roles in the tumorigenesis and progression of gastric cancer. METHODS： Immunohistochemistry SP method was used to detect the expression of Maspin, uPA and MMP-7 in tissues from gastric adenocarcinoma （n = 30）, signet-ring cell carcinoma （n = 30）, and normal gastric mucosa （n = 20）. RESULTS： The positive rates of Maspin, uPA and MMP-7 were 50%, 70% and 80%, respectively, in gastric adenocarcinoma, and they were 46.7%, 76.7% and 90%, respectively, in signetring cell carcinoma. However, the positive rates of Maspin, uPA, and MMP-7 were 90%, 35%, and 30%, respectively, in normal gastric mucosa. Maspin, uPA, and MMP-7 expression were significantly different between tissues from carcinoma and normal mucosa （P 〈 0.05 or P 〈 0.01）. Maspin expression was significantly related to the depth of invasion and lymph node metastasis, but not to tumor size and TNM staging. The expression of uPA and MMP-7 were markedly related to the depth of invasion, lymph node metastasis and TNM staging, but not to tumor size. Maspin expression had a negative correlation with uPA and MMP-7 （P = 0.012, r = -0.322; P = 0.008, r = -0.341）, while uPA expression had a positive correlation with MMP-7 （P = 0.034, r = 0.274）. CONCLUSION： Down-regulated expression of Maspin and up-regulated expression of uPA and MMP-7 play important roles in the invasion and metastasis of gastric carcinoma. They may serve as effective markers of reflecting the biopathological behaviors of gastric carcinoma.