应用基因表达谱芯片筛选胃腺癌相关基因

Screening of genes differentially expressed in gastric adenocarcinoma by gene expression profiling technique

目的:利用基因芯片技术筛选胃腺癌组织和癌旁正常组织间的差异表达基因．方法:分别抽取胃腺癌组织和癌旁正常组织的总RNA．采用逆转录的方法,制成cDNA链, 并以两种荧光Cy5和Cy3标记后作为探针,与含有14 784条人类14KcDNA基因表达谱芯片进行杂交．以Agilent荧光扫描仪扫描芯片上两种荧光信号,并用计算机处理和分析．结果:在14 784条基因中,4例胃腺癌组织和癌旁正常组织共同差异表达基因29条,其中上调基因10条,下调基因19条,上调的基因中有2 条功能信息不明．结论:胃腺癌发生过程中有多基因的参与,胃腺癌与癌旁正常组织共同差异表达的29条基因可能与胃癌的发生、发展有关．

AIM： To investigate the genes differently expressed between gastric adenocarcinoma and normal mucosal tissues by eDNA microarray technique. METHODS： The total RNA was extracted from gastric adenocarcinoma and normal mucosal tissues and then purified. The cDNA was obtained by reverse transcription polymerase chain reaction （RT-PCR）, and then labeled with Cy5 and Cy3 fluorescence as probes, which were hybridized with gene chip containing 14 784 human 14KcDNA. Subsequently, the signal images were scanned by Agilent fluorescence scanner and analyzed by Pix QuantArray software. RESULTS： Of the 14 784 target genes, 29 genes were screened out for differences in gene expression level between gastric adenocarcinoma and normal gastric mucosa. Ten of the 29 genes were up-regulated, of which 2 were with unknown function, and 19 were down-regulated. CONCLUSION： Multiple genes take part in the carcinogenesis of gastric adenocarcinoma. The 29 gene differentially expressed may be involved in the occurrence and development of gastric cancer.