摘要
目的:探讨RhoC蛋白表达和肝细胞癌 (hepatocellular carcinoma,HCC)临床病理的关系及其和微血管生成之间的联系.方法:应用免疫组织化学法,检测49例HCC、 22例肝癌转移灶组织、9例正常肝组织内 RhoC蛋白表达和微血管密度(microvessel density,MVD),并将检测结果与患者的临床资料进行统计分析.结果:RhoC蛋白在9例正常肝组织中有1例呈阳性表达,占11.1%,而在49例HCC中有36 例阳性表达,占73.5%,22例肝癌转移灶中有 18例呈阳性表达,占81.8%,正常肝组织组与另两组比较有显著性差异(P<0.01);RhoC蛋白表达强度与年龄、性别、肝癌大小、AFP 表达量、HBsAg是否阳性及有无包膜无关 (P>0.05),而与肝癌分化程度、有无侵袭转移有显著关系(X2=8.037,P=0.005;X2=7.335, P=0.007).肝癌、肝癌转移灶中MVD显著高于正常肝组织(H=4.564,P<0.01;H=4.442. P<0.01).RhoC阳性表达的肝癌MVD值为93.3 ±25.0/mm3,阴性表达的肝癌MVD值为55.7 ±23.4/mm3,前者明显高于后者(t=4.715, P<0.01).结论:RhoC在HCC组织中的表达上调与其血管生成和侵袭转移有关.
AIM: To explore the expression of RhoC protein and its relations with rnicrovessel density (MVD) in hepatocellular carcinoma (HCC).
METHODS: Immunohistochemical staining was used to detect the expression of RhoC protein and MVD in the tissues from HCC (n = 49), metastatic focus of HCC and normal liver (n = 9). The relationship between the RhoC expression and pathological features as well as MVD was evaluated.
RESULTS: The positive rate of RhoC expression was 73.5% (36/49) in HCC, 81.8% (18/22) in the metastatic focus of HCC and 11.1% (1/9) in normal liver tissues, and there was significant difference between the former two and the latter (P 〈 0.01). The expression of RhoC protein in HCC was not correlated with the age, gender of patients, tumor size, α-fetoprotein expression, HBsAg and tumor encapsulation (P 〉 0.05), but significantly correlated with the Edmondson grading (χ^2= 8.037, P = 0.005) and tumor metastasis (χ^2 = 7.335, P = 0.007). The value of MVD in HCC, metastatic focus of HCC was markedly higher than that in the normal tissues (H = 4.564, P 〈 0.01; H = 4.442, P 〈 0.01). Meanwhile, the value of MVD in HCC with positive and negative expression of RhoC was 93.3 ± 25.0/mm^3 and 55.7 ± 23.4/mm^3, respectively, and there was notable difference between them (P 〈 0.01).
CONCLUSION: RhoC expression may be a good marker in the identification of HCC patients with high risks in the angiogenesis, invasion and metastasis.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第7期671-675,共5页
World Chinese Journal of Digestology
关键词
肝癌
转移
RHOC蛋白
微血管密度
Hepatocellular carcinoma
Metastasis
RhoC
Microvessel density