何首乌对Aβ1-40诱导的大鼠海马神经元内BDNF表达的影响

Effect of polygonum multiflorum thunb on BDNF expression in rat hippocampus induced by amyloid beta-protein(Aβ) 1-40

目的:探讨Aβ1-4 0对海马CA 1区神经元脑源性神经营养因子(brain-derived neurotroph icfactor,BDNF)表达的影响,以及何首乌对其的干预作用。方法:右侧海马微量注射Aβ1-4 0,观察海马BDNF的表达变化;并使用何首乌浸膏对大鼠模型进行干预治疗。动物存活3 0 d后,用“Y”迷宫检测各组动物学习和记忆能力;采用免疫组织化学方法和W estern-b lot检测海马CA 1区神经元BDNF的表达;应用尼氏染色法观察海马CA 1区神经元数目和形态。结果:Aβ1-4 0能够导致大鼠学会躲避电刺激所需的“尝试次数”明显增加;BDNF蛋白的表达明显下降,并可以在海马观察到Aβ1-4 0的沉积;CA 1区的神经元出现胞体肿胀、排列散乱等形态改变,且使用何首乌对动物模型干预治疗3 0 d后,大鼠的躲避电刺激所需的“尝试次数”明显下降;海马CA 1区神经元BDNF表达明显上调。结论:使用Aβ1-4 0在体内能够下调海马CA 1区神经元BDNF表达。何首乌干预治疗3 0 d,能够改善动物的学习和记忆能力,并能减少Aβ1-4 0对海马CA 1区神经元BDNF表达的抑制作用。

Objective To explore the effect of polygonum multiflorum thunb （PMT） on the expression of brain-drived neurtrophic factor （BDNF） in hippocampus CA1 in rats induced by Aβ1- 40. Methods The rat model was established by injecting Aβ1-40 into the right dorsal hippocampus in rats, and their behavior was observed by Y-maze. The morphological changes of rat hippocampus were examined by the Nissl staining. The expression of BDNF in hippocampus CA 1 neurons was also pression of BDNF in hippocampus CA1 were obviously reduced. The aggregation of Aβ1-40 in hippocampus and the morphological changes of CA 1 hippocampus were found. After the treatment with PMT for 30 days, the times learning how to elude electric stimulation were obviously reduced, while the expression of BDNF in hippocampus CA 1 was also increased. Conclusion PMT can reverse the down-regulation of BDNF expression in hippocampus CA 1 induced by Aβ1-40.