多发性骨髓瘤抗原致敏树突状细胞介导的体外抗瘤活性

Specific cell immune response mediated by dendritic cells in multiple myeloma patients in vitro

目的:探讨多发性骨髓瘤KM 3细胞株冻融抗原和弱酸洗脱抗原肽负载树突状细胞(DC)后诱导的特异性细胞毒性T淋巴细胞(CTL)对KM 3细胞的杀瘤作用。方法:联合GM-CSF,IL-4和TNF-α,体外诱导单核细胞生成DC,并使其致敏KM 3特异性冻融抗原或酸洗脱抗原,然后与自体T淋巴细胞共同培养3 d,生成特异性CTL,并采用MTT法检测其对KM 3细胞的杀伤率。结果:在细胞因子作用下,体外培养的外周血单个核细胞可诱导分化为成熟的DC;应用KM 3肿瘤抗原致敏DC,诱导生成的CTL对KM 3肿瘤细胞具有较强的杀伤效应。结论:KM 3冻融抗原和酸洗脱抗原激发的DC与自体T淋巴细胞孵育能诱生抗KM 3细胞特异性CTL。

Objective To explore KM3 multiple myeloma （MM） cell line specific cytotoxic T lymphocyte （CTL） response in vitro mediated by autologous dendritic cells （ DCs ） aroused by KM3 cells＇ lysates and acid-eluted peptides. Methods Monocytes were isolated from the peripheral blood of healthy individuals and MM patients, and were cultured in serum medium with IL-4, GM-CSF, and TNF-α to generate DCs. These DCs were pulsed by KM3 cells＇ lysates or the acid-eluted pep- tides, then incubated with autologous T lymphocytes for 3 days to induce KM3 cell antigen specific CTL. MTT assay was performed to examine the specific KM 3 cells＇ lysing ability of CTL. Results DCs were generated in peripheral blood monocytes cultured in the serum medium containing GM-CSF, IL-4, and TNF-ct. Autologous T lymphocytes induced by IL-2 and DCs pulsed with KM3 cells＇ lysates or the acid-eluted peptides showed strong killing effect on KM3 cells. Conclusion The DCs pulsed by KM3 cells＇ lysates or the acid-eluted peptides incubated with autologous T lymphocytes can induce KM3 cell antigen specific CTL.