形觉剥夺性近视视网膜细胞的凋亡及c-myc的表达

Apoptosis and c-myc protein expression in the retinal of form-deprivation myopia

目的:研究形觉剥夺性近视眼(FDM)中视网膜细胞的凋亡及c-myc蛋白在视网膜的表达。方法:孵化2 d的雏鸡,右眼眼睑缝合4,8,1 2周后检影验光,眼底视网膜照片和光镜观察,TUNEL(TdT-m ed iated b iotin-dUTP n ick-end labelling,TUNEL)检测及流式细胞术检测视网膜的凋亡细胞,免疫组织化学法及流式细胞术定量测定c-myc蛋白的表达。结果:1 2周时缝合眼中出现漆裂纹样眼底改变,视网膜内、外核层发现凋亡细胞,出现明显凋亡峰。8,1 2周视网膜c-myc蛋白表达高于对照组。结论:鸡眼近视形成伴眼底改变时,视网膜细胞出现凋亡,c-myc在近视视网膜细胞的凋亡过程中发挥重要作用。

Objective To study the apoptosis of retina and the expression of c-myc protein in form-deprivation myopia. Methods Two-day-old chickens were sutured with right eyelid for 4,8 and 12 weeks. After measurement of refracation, the eyeballs were observed by light microscope and taken photos. Retinal apoptotic cells were measured by TUNEL staining and flow cytometry. C-myc protein were examined by immunohistochemistry and flow cytometry. Results Lacquer crack lesions were found in sutured eyes at 12 weeks. Apoptotic ceils were observed in retinal outer and inner nuclear layer of the sutured eyes at 12 weeks and obvious peak of apoptosis was observed in sutured eyes at 12 weeks. The expression of c-myc protein was significantly more than control eyes at 8 and 12 weeks. Conclusion The apoptosis of retinal was present in form-deprivation myopia with the degeneration of retina. C-myc protein plays an important role in retinal apoptosis of myopia.